Absence of detection of xanthomonas axonopodis pv. malvacearum in cotton seeds used in the Northeast Region of Mato Grosso do Sul in a three-based crop season study
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Data
2014-03-01
Autores
Theodoro, Gustavo de Faria
Correia, Hectory de Castro
Silva, Rafael Rodrigues da
Chumpati, Andre Augusto
Cortina, Josiane Vogel
Maringoni, Antonio Carlos [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Univ Federal Uberlandia
Resumo
The aim of this work was to evaluate the presence of the causal agent of angular leaf spot, Xanthomonas axonopodis pv. malvacearum, in 38 cotton seeds samples with or without linter used in the northeast region of Mato Grosso do Sul, Brazil, during the crop seasons of 2008/09, 2009/10 e 2010/11. It was used the semi selective culture media MSSXAN (beef extract - 3 g, peptone - 5 g, soluble starch - 10 g, sucrose - 5g; Tween 80 - 10 mL; CaCl2 - 0.25 g; crystal violet solution at 1% - 150 mL; cephalexin - 50 mg; chlorothalonil - 10 mg; methyl thyophanate - 10 mg; agar - 15 g; sterile distilled water - 1000 ml) for the isolation of the bacteria and the suspect colonies of being X. axonopodis pv. malvacearum were purified and submitted to the Gram stain, potassium hydroxide and pathogenicity tests in the leaves of cotton plants of the susceptible genotype NC 53345. The presence of X. a. pv. malvacearum was not detected in the evaluated seed samples.
Descrição
Palavras-chave
Gossypium hirsutum, Seed pathology, Dissemination
Como citar
Bioscience Journal. Uberlandia: Univ Federal Uberlandia, v. 30, n. 2, p. 326-331, 2014.