Identification of Corynebacterium spp. isolated from bovine intramammary infections by matrix-assisted laser desorption ionization-time of flight mass spectrometry
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Data
2014-09-17
Autores
Goncalves, Juliano Leone
Tomazi, Tiago
Barreiro, Juliana Regina
Campos Braga, Patricia Aparecida de
Ferreira, Christina Ramires
Araujo Junior, Joao Pessoa [UNESP]
Eberlin, Marcos Nogueira
Santos, Marcos Veiga dos
Título da Revista
ISSN da Revista
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Editor
Elsevier B.V.
Resumo
Corynebacterium species (spp.) are among the most frequently isolated pathogens associated with subclinical mastitis in dairy cows. However, simple, fast, and reliable methods for the identification of species of the genus Corynebacterium are not currently available. This study aimed to evaluate the usefulness of matrix-assisted laser desorption ionization/mass spectrometry (MALDI-TOF MS) for identifying Corynebacterium spp. isolated from the mammary glands of dairy cows. Corynebacterium spp. were isolated from milk samples via microbiological culture (n = 180) and were analyzed by MALDI-TOF MS and 16S rRNA gene sequencing. Using MALDI-TOF MS methodology, 161 Corynebacterium spp. isolates (89.4%) were correctly identified at the species level, whereas 12 isolates ( 6.7%) were identified at the genus level. Most isolates that were identified at the species level with 16 S rRNA gene sequencing were identified as Corynebacterium bovis (n = 156; 86.7%) were also identified as C. bovis with MALDI-TOF MS. Five Corynebacterium spp. isolates (2.8%) were not correctly identified at the species level with MALDI-TOF MS and 2 isolates (1.1%) were considered unidentified because despite having MALDI-TOF MS scores >2, only the genus level was correctly identified. Therefore, MALDI-TOF MS could serve as an alternative method for species-level diagnoses of bovine intramammary infections caused by Corynebacterium spp. (C) 2014 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Subclinical mastitis, Corynebacterium spp., Mass spectrometry
Como citar
Veterinary Microbiology. Amsterdam: Elsevier Science Bv, v. 173, n. 1-2, p. 147-151, 2014.