Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology
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Data
2015-06-01
Autores
Mendes, N. J. [UNESP]
Cruz, V. P. [UNESP]
Mendonça, Fernando Fernandes [UNESP]
Pardo, B. G.
Coelho, R.
Ashikaga, F. Y. [UNESP]
Camargo, S. M. [UNESP]
Martinez, P.
Oliveira, C. [UNESP]
Santos, M. N.
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Editor
Springer
Resumo
Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark (Carcharhinus longimanus) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in population screening. The genetic diversity of these loci was assessed in 28 individuals and a total of 35 alleles were identified, ranging from four to eight alleles per locus (5.9 alleles on average). Cross-species amplification of these markers was successful in five loci for C. acronotus, C. perezi and Galeocerdo cuvier. The developed microsatellite markers will be useful in analyzing the population genetics structure of the oceanic whitetip shark and other Carcharhinidae species.
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Palavras-chave
Conservation, Population genetics, Next generation sequencing, Carcharhinus
Como citar
Conservation Genetics Resources. Dordrecht: Springer, v. 7, n. 2, p. 585-589, 2015.