Involvement of major components from sporothrix schenckii cell wall in the caspase-1 activation, nitric oxide and cytokines production during experimental sporotrichosis
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Data
2015-02-01
Autores
Goncalves, Amanda Costa [UNESP]
Geraldo Maia, Danielle Cardoso [UNESP]
Ferreira, Lucas Souza [UNESP]
Silva Monnazzi, Luis Gustavo [UNESP]
Alegranci, Pamela [UNESP]
Polesi Placeres, Marisa Campos [UNESP]
Batista-Duharte, Alexander [UNESP]
Carlos, Iracilda Zeppone [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Resumo
Sporotrichosis is a chronic infection caused by the dimorphic fungus Sporothrix schenckii, involving all layers of skin and the subcutaneous tissue. The role of innate immune toll-like receptors 2 and 4 in the defense against this fungus has been reported, but so far, there were no studies on the effect of cell wall major components over the cytosolic oligo-merization domain (NOD)-like receptors, important regulators of inflammation and responsible for the maturation of IL-1 beta and IL-18, whose functions are dependents of the caspase-1 activation, that can participate of inflammasome. It was evaluated the percentage of activation of caspase-1, the production of IL-1 beta, IL-18, IL-17, IFN-gamma and nitric oxide in a Balb/c model of S. schenckii infection. It was observed a decreased activity of caspase-1 during the fourth and sixth weeks of infection accompanied by reduced secretion of the cytokines IL-1 beta, IL-18 and IL-17 and high production of nitric oxide. IFN-gamma levels were elevated during the entire time course of infection. This temporal reduction in caspase-1 activity coincides exactly with the reported period of fungal burden associated with a transitory immunosuppression induced by this fungus and detected in similar infection models. These results indicate the importance of interaction between caspase-1, cytokines IL-1 beta and IL-18 in the host defense against S. schenckii infection, suggesting a participation the inflammasome in this response.
Descrição
Palavras-chave
Cytokines, Caspase-1, Nitric oxide, Sporothrix schenckii
Como citar
Mycopathologia, v. 179, n. 1-2, p. 21-30, 2015.