Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma
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Data
2012-04-01
Autores
Silveira, Sara Martoreli
Domingues, Maria Aparecida Custódio [UNESP]
Butugan, Ossamu
Brentani, Maria Mitzi
Rogatto, Silvia Regina [UNESP]
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Wiley-Blackwell
Resumo
Background To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions.Methods. High-resolution comparative genomic hybridization (HR-CGH) was used in laser-captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real-timereverse transcription polymerase chain reaction (qRT-PCR) in 15 cases.Results. Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM, CDH1, CTNNB1, FGF18, and SUPT16H. A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down-expression in endothelial cells.Conclusions. A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB, FGF18, and SUPT16H were identified as potential molecular markers in JNA. (C) 2011 Wiley Periodicals, Inc. Head Neck 34: 485-492, 2012
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juvenile nasopharyngeal angiofibromas, quantitative real-time RT-PCR, chromosomal imbalances, high-resolution comparative genomic hybridization, tumor microenvironment
Como citar
Head and Neck-Journal For The Sciences and Specialties of The Head and Neck. Malden: Wiley-blackwell, v. 34, n. 4, p. 485-492, 2012.