Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles
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Data
2015
Autores
Rodrigues, Phamilla Gracielli Sousa
Campos de Menezes, Priscila Fernanda
Fujita, Alessandra Keiko Lima
Escobar, André [UNESP]
Barboza de Nardi, Andrigo [UNESP]
Kurachi, Cristina
Bagnato, Vanderlei S.
Título da Revista
ISSN da Revista
Título de Volume
Editor
2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Resumo
Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.
Descrição
Palavras-chave
Ppix production, Aminolevulinic acid, Microneedle rollers, Photodynamic therapy, Porcine skin
Como citar
Journal Of Biophotonics, v. 8, n. 9, p. 723-729, 2015.