A modular approach to study protein adsorption on surface modified hydroxyapatite
Nenhuma Miniatura disponível
Data
2015
Autores
Ozhukil Kollath, Vinayaraj [UNESP]
Van den Broeck, Freya
Fehér, Krisztina
Martins, José C.
Luyten, Jan
Traina, Karl
Mullens, Steven
Cloots, Rudi
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Resumo
Biocompatible inorganic nano- and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the protein adsorption capacity of hydroxyapatite (HA) powder. The adsorption capacity of bovine serum albumin as a model protein has been studied with respect to the surface modifications. The selected linker molecules (lysine, arginine, and phosphoserine) can influence the adsorption capacity by changing the electrostatic nature of the HA surface. Qualitative and quantitative analyses of linker-molecule interactions with the HA surface have been performed by using NMR spectroscopy, zeta-potential measurements, X-ray photoelectron spectroscopy, and thermogravimetric analyses. Additionally, correlations to theoretical isotherm models have been calculated with respect to Langmuir and Freundlich isotherms. Lysine and arginine increased the protein adsorption, whereas phosphoserine reduced the protein adsorption. The results show that the adsorption capacity can be controlled with different functionalization, depending on the protein-carrier selections under consideration. The scientific knowledge acquired from this study can be applied in various biotechnological applications that involve biomolecule-inorganic material interfaces.
Descrição
Palavras-chave
Adsorption, Amino acids, NMR spectroscopy, Proteins, Surface chemistry
Como citar
Chemistry (weinheim an der Bergstrasse, Germany), v. 21, n. 29, p. 10497-10505, 2015.