In vitro study of cyclosporine A 0.05 % on primary and recurrent pterygium fibroblasts
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Data
2015-08-11
Autores
Viveiros, Magda Massae Hata [UNESP]
Kakizaki, Fabiano Yutaka [UNESP]
Hércules, Laura Almeida [UNESP]
Padovani, Carlos Roberto [UNESP]
Candeias, João Manuel Grisi [UNESP]
Schellini, Silvana Artioli [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
International Ophthalmology
Resumo
To compare the cyclosporine 0.05 % exposure effect on fibroblasts from primary and recurrent pterygium. Primary culture of fibroblasts from primary and recurrent pterygium was performed until the third passage, which was exposed to cyclosporine 0.05 % in a group and the other remaining unexposed (control group), in triplicates. After 3, 6, 12, and 17 days of exposure the viable cell counting was performed by hemocytometer. The results were statistically analyzed using the technique of analysis of non-parametric variance model for repeated measures with three factors. There was a significant reduction in both fibroblast proliferation, in primary as in the recurrent pterygium cultures exposed to cyclosporine when compared not exposed cultures, with statistical significance (P < 0.05). Comparing primary and recurrent pterygium that received the drug, there was no significant difference in cell proliferation in relation to primary or recurrent pterygium. Cyclosporine 0.05 % is effective in inhibiting fibroblast proliferation in culture, both in primary and as in recurrent pterygium.
Descrição
Palavras-chave
Cyclosporine, Pterygium, Fibroblasts, In vitro
Como citar
International Ophthalmology, 2015.