Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale
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Data
2015-09-15
Autores
Pedrassani, Daniela
Nascimento, Adjair Antonio do [UNESP]
André, Marcos Rogério [UNESP]
Machado, Rosangela Zacarias [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B. V.
Resumo
An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
Descrição
Palavras-chave
Dioctophyma, Dogs, Elisa test, Serological diagnosis
Como citar
Veterinary Parasitology, v. 212, n. 3-4, p. 435-438, 2015.