Estudo genético da característica fibra em cana-de-açúcar
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Data
2016-02-24
Autores
Kettener, Karine [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Os resultados obtidos durante o desenvolvimento deste trabalho estão apresentados na forma de capítulos. O primeiro capítulo apresenta uma análise dos parâmetros genéticos de uma população F1 obtida a partir do cruzamento de duas variedades comerciais de cana-de-açúcar oriundas do programa de melhoramento do Centro de Tecnologia Canavieira, Piracicaba/SP. Também avaliamos marcadores moleculares microssatélites buscando uma relação entre os mesmos com as características agronômicas aqui estudadas (Peso do bolo úmido, Peso do bolo seco, Fibra, Lignina e Celulose). No segundo capítulo, o qual está em formato de artigo científico que será submetido para a revista Genome, com o título “A SNP genetic map constructed using restriction site-associated DNA sequencing approach for sugarcane”, apresenta o primeiro mapa genético com marcadores SNPs (Single nucleotide polymorphisms) obtidos via RADSeq utilizando a população F1 de mapeamento obtida a partir do cruzamento de uma variedade comercial dos Estados Unidos com S. spontaneum, oriundos do programa de melhoramento da Texas A&M AgriLife Research, Weslaco, TX, USA. Durante o doutorado realizei estágio na Texas A&M AgriLife Research, Weslaco, TX, USA, sob orientação do Dr. Jorge A. G. da Silva, onde foram realizadas as análises dos componentes lignocelulósicos e o screening de marcadores SSR. Também houve o estágio na Texas A&M AgriLife Genomics and Bioinformatics Service (Texas A&M University, College Station, TX, EUA), sob orientação do Dr. Charles Johnson, onde a genotipagem para a descoberta de SNPs foi realizada através do protocolo RadSeq. As análises de bioinformática para o mapeamento genético foi realizado em parceria com o Prof. Dr. Antonio Augusto Franco Garcia da ESALQ-USP, Piracicaba.
The results obtained during this work are presented in chapters. The first chapter shows an analysis of genetic parameters of an F1 population obtained from the crossing of two commercial varieties of sugarcane derived from the Sugar Cane Technology Center breeding program, Piracicaba / SP. We also screened microsatellites in this population seeking a relationship between them with agronomic traits like the weight of wet cane, dry cane weight, fiber, lignin and cellulose content. In the second chapter, which is in scientific paper format to be submitted to Genome journal, entitled "The SNP genetic map constructed using restriction site-associated DNA sequencing approach for sugarcane," presents the first genetic map with SNP markers (single nucleotide polymorphisms) obtained by RADSeq using the F1 population of mapping obtained from the crossing of a commercial variety of the United States with S. spontaneum, coming from the breeding program at Texas A & M AgriLife Research, Weslaco, TX, USA. During the doctoral stage at Texas A & M AgriLife Research, Weslaco, TX, USA, under the supervision of Dr. Jorge A. G. Silva, were carried out the analysis of lignocellulosic components and the screening of SSR markers. There was also a stage at Texas A & M AgriLife Genomics and Bioinformatics Service (Texas A & M University, College Station, TX, USA), under the supervision of Dr. Charles Johnson, where the genotyping for SNPs discovery was made by RadSeq protocol. The bioinformatics analysis for genetic mapping was conducted in partnership with Prof. Dr. Antonio Augusto Franco Garcia ESALQ-USP, Piracicaba.
The results obtained during this work are presented in chapters. The first chapter shows an analysis of genetic parameters of an F1 population obtained from the crossing of two commercial varieties of sugarcane derived from the Sugar Cane Technology Center breeding program, Piracicaba / SP. We also screened microsatellites in this population seeking a relationship between them with agronomic traits like the weight of wet cane, dry cane weight, fiber, lignin and cellulose content. In the second chapter, which is in scientific paper format to be submitted to Genome journal, entitled "The SNP genetic map constructed using restriction site-associated DNA sequencing approach for sugarcane," presents the first genetic map with SNP markers (single nucleotide polymorphisms) obtained by RADSeq using the F1 population of mapping obtained from the crossing of a commercial variety of the United States with S. spontaneum, coming from the breeding program at Texas A & M AgriLife Research, Weslaco, TX, USA. During the doctoral stage at Texas A & M AgriLife Research, Weslaco, TX, USA, under the supervision of Dr. Jorge A. G. Silva, were carried out the analysis of lignocellulosic components and the screening of SSR markers. There was also a stage at Texas A & M AgriLife Genomics and Bioinformatics Service (Texas A & M University, College Station, TX, USA), under the supervision of Dr. Charles Johnson, where the genotyping for SNPs discovery was made by RadSeq protocol. The bioinformatics analysis for genetic mapping was conducted in partnership with Prof. Dr. Antonio Augusto Franco Garcia ESALQ-USP, Piracicaba.
Descrição
Palavras-chave
Genetic maps, RADseq, SNP, Sugarcane