Estudo Químico e Biológico do extrato metanólico dos capítulos de Paepalanthus acanthophyllus Ruhland (Eriocaulaceae)
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Data
2016-08-17
Autores
Ignácio, Felipe Gregório [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Paepalanthus é um dos gêneros mais representativos da família Eriocaulaceae e consta na literatura que já foram isoladas naftopiranonas e flavonoides, substâncias com comprovadas atividades como antirradicalar, antiúlcera e mutagênica. Apesar dos estudos realizados até o momento com espécies de Eriocaulaceae, é importante destacar que ainda existe um grande número de espécies que não foram estudados química e biologicamente. Portanto, neste trabalho descrevemos o estudo químico e biológico do extrato metanólico dos capítulos de Paepalanthus acanthophyllus. A estratégia de fracionamento por HPLC-PDA em escala semipreparativa do extrato metanólico permitiu isolar as substâncias 6-metoxicanferol-3-O-(6”-p-cumaroil)-β-D-glucopiranosil-7-O-β-D-glucopiranosídeo, Pa2, 6-metoxicanferol-3-7-di-O-β-D-glucopiranosídeo,Pa1, 6-metoxicanferol-3-O-β-D-glucopiranosídeo, Pa3, Paepalantina-9-O-β-D-glucopiranosideo, Pa4 e a Paepalantina, Pa5. A padronização do extrato metanólico foi realizada por meio da quantificação dos derivados do canferol glicosilados (Pa1, Pa2 e Pa3) e do derivado da paepalantina glicosilada (Pa4) por HPLC-PDA. A metodologia permitiu avaliar os parâmetros de linearidade, limites de detecção e quantificação.Os limites de detecção (12,22 μg.mL-1) e quantificação (30,03 μg.mL-1) foram satisfatórios para as condições analisadas para os derivados do canferol. Para o derivado da paepalantina glicosilada observou-se os valores de limite de detecção 25,54μg.mL-1 e de quantificação 77,39μg.mL-1.Os teoresdas substânciasencontrados no extrato metanólicodos capítulosforam de 2,39 % (Pa1), 1,22 % (Pa2), 2,52 % (Pa3) e 8,38 % (Pa4). O teor de fenóis totais encontrados foi de 126,41 mg.g-1de extrato para os capítulos.Paralelamente foram avaliadas as atividades antimicrobiana do extrato metanólico dos capítulos de P. acanthophyllus frente a bacterias Gram-Positiva e Gram-negativa, sendo encontrados valores >1000 g.mL-1para todas as cepas avaliadas. Os valores para a avaliação da citotoxicidade utilizando o método do MTT para o extrato metanólico dos capítulos foi de IC50 = 34,81 µg.mL-1paraa linhagem MCF7 (cancro da mama humana), sendo considerado um valor promissor.
Paepalanthus is one of the most representative genus of the Eriocaulaceae family and it is known in literature that naphthopyranones and flavonoids were isolated. These substances are proven to have antiradical, antiulcer and mutagenic biological activities. Despite available studies of the Eriocaulaceae species, it is important to emphasize that there is still a large number of species that have not been studied chemically and biologically. Therefore, in this work we describe the chemical and biological study of the methanol extract of the capitulae from Paepalanthus acanthophyllus. The fractionation strategy by HPLC-PDA semipreparative scale of the methanolic extract allowed us to isolate substances 6-methoxykaempferol-3-O-(6"-p-coumaroyll)-β-D-glucopyranosyl-7-O-β-D-glucopyranoside, Pa2, 6- methoxyKaempferol-3-7-di-O-β-D-glucopyranoside,Pa1, 6- methoxyKaempferol-3-O-β-D-glucopyranoside, Pa3, Paepalantina-9-O-β-D-glucopyranoside, Pa4 and PaepalantinePa5. The standardization of the methanol extract was performed by quantification of kaempferol glycosides derivatives and paepalantina glycosylated by HPLC-PDA. The methodology allowed us to evaluate the parameters: linearity, limits of detection and quantification. The detection limits (12.22 μg.mL-1) and quantitation (30.03 μg.mL-1) were satisfactory for the conditions tested for the kaempferol derivatives. For the derivative of the glycosylated paepalantina, the detection limit (25.54 μg.mL-1) and quantization limit (77.39 μg.mL-1) were observed. The content of substances found in the methanolic extract of the capitulae were 2.39%(Pa1), 1.22% (Pa2), 2.52% (Pa3) and 8.38% (Pa4). The totalphenols content obtained was 126.41 mg.g-1 in the extract of the capitulae . At the same time we evaluated the antimicrobial activities of the methanolic extract of P. acanthophyllus against bacteria Gram-positive and Gram-negative, and observed the values > 1000 mg.mL-1 for all tested strains. The values for the assessment of cytotoxicity using MTT method for the methanolic extract of capitulae was IC50 = 34.81 μg.mL-1 strain against MCF7 (human breast cancer).It is considered a promising value.
Paepalanthus is one of the most representative genus of the Eriocaulaceae family and it is known in literature that naphthopyranones and flavonoids were isolated. These substances are proven to have antiradical, antiulcer and mutagenic biological activities. Despite available studies of the Eriocaulaceae species, it is important to emphasize that there is still a large number of species that have not been studied chemically and biologically. Therefore, in this work we describe the chemical and biological study of the methanol extract of the capitulae from Paepalanthus acanthophyllus. The fractionation strategy by HPLC-PDA semipreparative scale of the methanolic extract allowed us to isolate substances 6-methoxykaempferol-3-O-(6"-p-coumaroyll)-β-D-glucopyranosyl-7-O-β-D-glucopyranoside, Pa2, 6- methoxyKaempferol-3-7-di-O-β-D-glucopyranoside,Pa1, 6- methoxyKaempferol-3-O-β-D-glucopyranoside, Pa3, Paepalantina-9-O-β-D-glucopyranoside, Pa4 and PaepalantinePa5. The standardization of the methanol extract was performed by quantification of kaempferol glycosides derivatives and paepalantina glycosylated by HPLC-PDA. The methodology allowed us to evaluate the parameters: linearity, limits of detection and quantification. The detection limits (12.22 μg.mL-1) and quantitation (30.03 μg.mL-1) were satisfactory for the conditions tested for the kaempferol derivatives. For the derivative of the glycosylated paepalantina, the detection limit (25.54 μg.mL-1) and quantization limit (77.39 μg.mL-1) were observed. The content of substances found in the methanolic extract of the capitulae were 2.39%(Pa1), 1.22% (Pa2), 2.52% (Pa3) and 8.38% (Pa4). The totalphenols content obtained was 126.41 mg.g-1 in the extract of the capitulae . At the same time we evaluated the antimicrobial activities of the methanolic extract of P. acanthophyllus against bacteria Gram-positive and Gram-negative, and observed the values > 1000 mg.mL-1 for all tested strains. The values for the assessment of cytotoxicity using MTT method for the methanolic extract of capitulae was IC50 = 34.81 μg.mL-1 strain against MCF7 (human breast cancer).It is considered a promising value.
Descrição
Palavras-chave
Flavonoides, Citotoxicidade, Cromatografia Líquida de Alta Eficiência, Eriocaulaceae, Atividade Antifúngica, Flavonoids, Citotoxicity, High performance liquid chromatography, Eriocaulaceae, Antifungal activity