Aspectos celulares da remodelação tecidual da próstata de gerbilos frente à orquiectomia
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Data
2017-03-03
Autores
Castro, Nayara Fernanda da Costa [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A próstata é uma glândula acessória do sistema genital dos mamíferos cujo desenvolvimento ocorre sob influência de andrógenos, hormônios esteroides responsáveis por induzir a diferenciação e a maturação do sistema reprodutor masculino e por atuar no desenvolvimento das características sexuais secundárias masculinas. A privação dos andrógenos pode ocorrer por meio da orquiectomia (castração cirúrgica), a fim de suprimir os níveis de testosterona e promove, na glândula prostática, a atrofia das células epiteliais. E as células musculares lisas passam a atuar na adaptação do compartimento estromal que interage e contribui para a redução da atividade das estruturas epiteliais. Nesse compartimento é possível observar a presença de vários tipos celulares, como os macrófagos, que são responsáveis por atuar ativamente na homeostase do sistema reprodutivo. Dessa forma, o presente estudo teve por objetivo avaliar as modificações decorrentes da remodelação tecidual resultantes da privação de andrógenos na próstata ventral de gerbilos, com ênfase na distribuição e ocorrência dos macrófagos. Foram utilizados gerbilos (Meriones unguiculatus) machos adultos divididos nos grupos: controle e castrados (eutanasiados no 3º, 7º e 14º dia após a orquiectomia). O lobo ventral da próstata desses animais foi removido, pesado e processado para microscopia de luz. Foram realizadas análises estereológicas, morfométricas, sorológica, imunoistoquímicas e imunofluorescência. Os dados obtidos indicam que a castração promoveu o rearranjo dos diferentes tipos celulares e influenciou em alterações na proporção relativa dos compartimentos teciduais da próstata ventral. Assim, os resultados obtidos poderão contribuir para o maior entendimento da ação das células estromais no processo de remodelação tecidual frente a realização da orquiectomia, principalmente em gerbilos, em que os efeitos a curto prazo (três dias), ainda não tinham sido relatados.
The prostate is an accessory gland of the genital system of the mammals and its development occurs under the influence of androgens, steroid hormones wich are responsible for inducing the differentiation and maturation of the male reproductive system, operating in the development of the male secondary sex characteristics. Deprivation of androgens may occur through orchiectomy (surgical castration) in order to suppress testosterone levels. Orchiectomy promotes atrophy of the epithelial cells in the prostate gland and the smooth muscle cells are adapted to operate in the stromal compartment to reduce the epithelial structures activity. In the stromal compartment is possible to observe various cell types, including macrophages that work actively in the homeostasis of the reproductive system tissues. Thus, this study aims to evaluate the changes resulting from tissue remodeling resulting from androgen deprivation in the ventral prostate of gerbils, with emphasis on the distribution and occurrence of macrophages. Adult males gerbils (Meriones unguiculatus) was divided into groups: control and castrated (euthanized at 3 th, 7th and 14th days after orchiectomy). In these animals, the ventral lobe of the prostate was removed, weighed and processed for light microscopy. Stereological, morphometric, serological, immunohistochemistry and immunofluorescence analysis was performed. The data indicated that castration promoted a rearrangement of the different cell types and influenced in alterations in the relative proportion of the tissue compartments of the ventral prostate. Thus, the results may contribute to a better understanding of the stromal cells actions in the tissue remodeling process, especially in gerbils, in which the short-term effects (three days) had not been reported.
The prostate is an accessory gland of the genital system of the mammals and its development occurs under the influence of androgens, steroid hormones wich are responsible for inducing the differentiation and maturation of the male reproductive system, operating in the development of the male secondary sex characteristics. Deprivation of androgens may occur through orchiectomy (surgical castration) in order to suppress testosterone levels. Orchiectomy promotes atrophy of the epithelial cells in the prostate gland and the smooth muscle cells are adapted to operate in the stromal compartment to reduce the epithelial structures activity. In the stromal compartment is possible to observe various cell types, including macrophages that work actively in the homeostasis of the reproductive system tissues. Thus, this study aims to evaluate the changes resulting from tissue remodeling resulting from androgen deprivation in the ventral prostate of gerbils, with emphasis on the distribution and occurrence of macrophages. Adult males gerbils (Meriones unguiculatus) was divided into groups: control and castrated (euthanized at 3 th, 7th and 14th days after orchiectomy). In these animals, the ventral lobe of the prostate was removed, weighed and processed for light microscopy. Stereological, morphometric, serological, immunohistochemistry and immunofluorescence analysis was performed. The data indicated that castration promoted a rearrangement of the different cell types and influenced in alterations in the relative proportion of the tissue compartments of the ventral prostate. Thus, the results may contribute to a better understanding of the stromal cells actions in the tissue remodeling process, especially in gerbils, in which the short-term effects (three days) had not been reported.
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Palavras-chave
Próstata, Orquiectomia, Macrófagos, Prostate, Orchiectomy, Macrophages