Análise histológica, imunoistoquímica e por micro-ct do processo de reparo alveolar de ratos orquiectomizados submetidos à terapia com teriparatida
Carregando...
Data
2017-04-04
Autores
Puttini, Igor de Oliveira [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Objetivos: Avaliar a influência da teriparatida, administrada sistemicamente, nas características morfológicas e estruturais do osso reparacional formado durante o processo de reparo alveolar em ratos com osteoporose induzida (orquiectomizados). Materiais e Métodos: Foram utilizados 48 ratos divididos 3 em grupos discriminados a seguir: 16 ratos orquiectomizados tratados com teriparatida, 16 ratos orquiectomizados sem tratamento e 16 ratos sham. Deste modo, dividindo-se 8 ratos para análise histológica e para a análise imuno-histoquímica que foi feita através da expressão das proteínas TRAP, osteocalcína, e WNT, com período de eutanásia em 42 dias (tecidos descalcificados), e 8 ratos para análise microtomográfica (tecidos calcificados), nesta análise o período de eutanásia foi de 60 dias pós exodôntico. Os dados quantitativos foram submetidos ao teste de normalidade e este indicou um teste estatístico mais adequado (paramétrico VS não paramétrico). Foi adotado para todos os testes nível de significância de 5%. Resultados: A análise histológica mostrou melhores padrões de neoformação óssea para o grupo ORQTRAT com maior quantidade de tecido mineralizado e pouco tecido conjuntivo. Os resultados imunohistoquímicos novamente mostraram um padrão de reparo melhor no grupo ORQTRAT com marcações mais intensas de wnt e osteocalcína e marcações mais fracas de trap. Aos resultados da microtomografia novamente o grupo ORQTRAT se demostrou melhor que os demais, com maior volume ósseo (BV), porcentagem de trabeculado ósseo (BV/TV), espessura do trabeculado ósseo (Tb.Th) e separação entre as trabéculas (Tb.Sp). E, menor número (Tb.N) e menor porosidade total óssea (Po.Tot) quando comparado ao grupo sem tratamento.
Objectives: To evaluate the influence of teriparatide, systemically administered, on the morphological and structural characteristics of the reparative bone formed during the alveolar repair process in rats with induced osteoporosis (orchiectomized). Materials and Methods: Forty-eight rats were divided into three groups: 16 orchiectomized rats treated with teriparatide, 16 unqualified orchiectomized rats and 16 sham rats. Thus, 8 rats were divided for histological analysis and for Immunohistochemical analysis, which was done by the expression of TRAP proteins, osteocalcin, and WNT, with euthanasia at 42 days (decalcified tissues), and 8 rats for analysis for microtomographic analysis (calcified tissues). In this analysis, the euthanasia period was at 60 days after the extraction surgery. The quantitative data were submitted to the normality test and this indicated the most adequate statistical test (parametric vs non-parametric). The significance level of 5% was adopted for all tests. Results: Histological analysis showed better patterns of bone neoformation for the ORQTRAT group with greater amount of mineralized tissue and little connective tissue. The immunohistochemical results again showed a better repair pattern in the ORQTRAT group with more intense markings of wnt and osteocalcin and weaker trap markings. To the results of the microtomography again the ORQTRAT group showed better than the others, with higher bone volume (BV), percentage of trabecular bone (BV / TV), thickness of trabecular bone (Tb.Th) and separation between trabeculae (Tb.Sp). And, lower number (Tb.N) and lower total bone porosity (Po.Tot) when compared to the non-treated group. Conclusions: It was possible to conclude that the treatment with Teriparatide improved important parameters that determine the quality of reparative bone tissue even in the presence of the established systemic condition in the animals.
Objectives: To evaluate the influence of teriparatide, systemically administered, on the morphological and structural characteristics of the reparative bone formed during the alveolar repair process in rats with induced osteoporosis (orchiectomized). Materials and Methods: Forty-eight rats were divided into three groups: 16 orchiectomized rats treated with teriparatide, 16 unqualified orchiectomized rats and 16 sham rats. Thus, 8 rats were divided for histological analysis and for Immunohistochemical analysis, which was done by the expression of TRAP proteins, osteocalcin, and WNT, with euthanasia at 42 days (decalcified tissues), and 8 rats for analysis for microtomographic analysis (calcified tissues). In this analysis, the euthanasia period was at 60 days after the extraction surgery. The quantitative data were submitted to the normality test and this indicated the most adequate statistical test (parametric vs non-parametric). The significance level of 5% was adopted for all tests. Results: Histological analysis showed better patterns of bone neoformation for the ORQTRAT group with greater amount of mineralized tissue and little connective tissue. The immunohistochemical results again showed a better repair pattern in the ORQTRAT group with more intense markings of wnt and osteocalcin and weaker trap markings. To the results of the microtomography again the ORQTRAT group showed better than the others, with higher bone volume (BV), percentage of trabecular bone (BV / TV), thickness of trabecular bone (Tb.Th) and separation between trabeculae (Tb.Sp). And, lower number (Tb.N) and lower total bone porosity (Po.Tot) when compared to the non-treated group. Conclusions: It was possible to conclude that the treatment with Teriparatide improved important parameters that determine the quality of reparative bone tissue even in the presence of the established systemic condition in the animals.
Descrição
Palavras-chave
Osso e ossos, Teriparatida, Homens, Osteoporose