Avaliação das propriedades morfo-fisicoquimica e biológica de diferentes acidos hialurônicos (ahs). Estudo in vivo e in vitro
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Data
2017-09-20
Autores
Oliveira, Morgana Regina Mendonça de
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo desse estudo foi realizar a caracterização morfo-fisicoquimica e avaliar a resposta histopatológica e a biodegradação de AH indicados para preenchimento dérmico: DMC-Facial 12,5% (D12) e RennovaFill (RF) e aumento volumétrico de tecido: DMC-Facial 15% (D15) e RennovaLift (RL). A caracterização foi realizada por microscopia eletrônica de varredura (MEV), espéctroscopia dispersiva de raios X (EDX) e infra vermelha com transformada de Fourier (FT-IR) e reometria dinâmica oscilatória. Para avaliação da resposta histopatológica e da biodegradação foi implantado 0,1 mL de cada AH no plano sub-dérmico do dorso de 25 ratos Hostsman, aleatóriamente distribuidos em 5 grupos (n=5), de acordo com o tempo de implantação dos AH (7, 14, 30, 60 e 120 dias). Um examinador experiente (HP), calibrado e cego para os AHs utilizados analisou os cortes. Modelo loglinear e teste de quiquadrado (χ2) foram utilizados para avaliar a associação entre os AH e a intensidade da resposta histopatológica e biodegradação para p<0,05. A hipótese nula (H0) foi rejeitada pois tanto na caracterização morfo-fisicoquimica quanto na avaliação da resposta histopatológica e da biodegradação mostraram diferenças signficatica entre os Ahs testados. Pode-se concluir que: 1) AH com a mesma indicação clínica diferem tanto nas características morfo-fisicoquimica com na resposta inflamatória provocada e no padrão de biodegradação. 2) O nível de resposta histopatológica depende do AH utilizado. 3) Uma morfologia estrutural características de D12 e D15, formada por câmaras separadas por septos finos, com bordas afiladas e irregulares, provoca maior resposta inflamatória, 4) Todos os AH testados estimularam a neo-formação de fibroblasto e colágeno. 5) A biodegradação dos AH é inversamente proporcional a taxa de reticulação.
The aim of this study was to perform the morpho-physicochemical characterization and to evaluate the histopathological response and biodegradation of AH indicated for dermal filling: DMC-Facial 12.5% (D12) and RennovaFill (RF) and tissue lifting: DMC-Facial 15% (D15) and RennovaLift (RL). The characterization was performed by scanning electron microscopy (SEM), X-ray dispersive (EDX) and Fourier transform infra red spectroscopy (FT-IR) and oscillatory dynamic rheometry. To evaluate the histopathological response and biodegradation, 0.1 mL of each HA was implanted in the dorsal subdermal plane of 25 male Hostsman rats, randomly distributed in 5 groups (n = 5), according to the time of implantation of AH: 7, 14, 30, 60 and 120 days). Slides were analyzed by a experienced examiner (HP), calibrated and blinded for the AH used. Loglinear model and chi-square statistical test (χ2) were used to evaluate the association between AH and the intensity of histopathological response and biodegradation for p <0.05. Results: The null hypothesis (H0) was rejected because both the morpho-physicochemically characterization and the histopathological analysis showed significant differences between the tested AHs. It can be concluded that: 1) AH with the same clinical indication differ in the morpho-physicochemical characteristics, in the inflammatory response and in the biodegradation pattern. 2) The level of histopathological response depends on the AH used. 3) A structural morphology characteristic of D12 and D15, formed by chambers separated by thin septa, with sharp and irregular borders, causes a greater inflammatory response, 4) All HA tested stimulated the fibroblast and collagen neoformation. 5) The biodegradation of HA is inversely proportional to the crosslinking rate.
The aim of this study was to perform the morpho-physicochemical characterization and to evaluate the histopathological response and biodegradation of AH indicated for dermal filling: DMC-Facial 12.5% (D12) and RennovaFill (RF) and tissue lifting: DMC-Facial 15% (D15) and RennovaLift (RL). The characterization was performed by scanning electron microscopy (SEM), X-ray dispersive (EDX) and Fourier transform infra red spectroscopy (FT-IR) and oscillatory dynamic rheometry. To evaluate the histopathological response and biodegradation, 0.1 mL of each HA was implanted in the dorsal subdermal plane of 25 male Hostsman rats, randomly distributed in 5 groups (n = 5), according to the time of implantation of AH: 7, 14, 30, 60 and 120 days). Slides were analyzed by a experienced examiner (HP), calibrated and blinded for the AH used. Loglinear model and chi-square statistical test (χ2) were used to evaluate the association between AH and the intensity of histopathological response and biodegradation for p <0.05. Results: The null hypothesis (H0) was rejected because both the morpho-physicochemically characterization and the histopathological analysis showed significant differences between the tested AHs. It can be concluded that: 1) AH with the same clinical indication differ in the morpho-physicochemical characteristics, in the inflammatory response and in the biodegradation pattern. 2) The level of histopathological response depends on the AH used. 3) A structural morphology characteristic of D12 and D15, formed by chambers separated by thin septa, with sharp and irregular borders, causes a greater inflammatory response, 4) All HA tested stimulated the fibroblast and collagen neoformation. 5) The biodegradation of HA is inversely proportional to the crosslinking rate.
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Ácido hialurônico, Preenchedores dérmicos, Reologia, Hyaluronic acid, Dermal fillers, Rheology