Utilização do perfil bioquímico-sérico, incluindo proteínas de fase aguda, como método auxiliar diagnóstico e prognóstico em fêmeas caninas acometidas por mucometra e piometra
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Data
2017-11-24
Autores
Milena Friolani
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo neste estudo foi analisar a utilização das proteínas de fase aguda (PFA) e perfil bioquímico-sérico como um método para realizar diagnóstico diferencial precoce de mucometra e piometra. Coletaram-se amostras do endométrio de fêmeas caninas hígidas na fase de diestro, sem qualquer alteração ou afecção no trato reprodutor ou em outros sistemas (Grupo diestro: Grupo 1, n=39) e amostras do endométrio de fêmeas caninas acometidas por mucometra (Grupo mucometra: Grupo 2, n=43), e fêmeas caninas acometidas por piometra (Grupo piometra, Grupo 3 ao 6, n=118), durante ovariohisterectomias (OH) eletivas ou terapêuticas. Os dados também foram comparados entre os grupos categorizados segundo a classificação ASA (American Society of Anesthesiologists). Realizou-se anamnese do histórico reprodutivo detalhado, exame físico, exame de macroscopia vulvar, citologia vaginal, exames hematológicos e bioquímicos, e dosagem sérica de progesterona e estradiol, exames de imagem (radiografia e ultrassonografia abdominal). A concentração de proteína total do soro sanguíneo foi determinada pelo método do biureto. As leituras das amostras foram realizadas em espectrofotômetro semi-automático (Labquest, Labtest, Belo Horizonte, Minas Gerais, Brasil), com comprimentos de onda específicos para o teste. Para o fracionamento proteico utilizou-se a técnica SDS-PAGE. Verificou-se que conforme a evolução do quadro inflamatório, os níveis séricos das proteínas de fase aguda, ceruloplasmina, transferrina, albumina (p<0,0001), IgGCP (p<0,0001), haptoglobina (p<0,0001), alfa glicoproteína ácida (p<0,0001), IgGCL (p<0,0001) e as identificadas pelo seu peso atômico 44.000 Da, 40.000 Da (p=0,0391) e 23.000 Da (p=0,0031), sofreram alterações significantes, auxiliando no prognóstico precoce e até mesmo em terapêuticas mais eficazes.
The objective of this study was to analyze the use of acute phase proteins (PFA) and biochemical-serum profile as a method to perform early differential diagnosis of mucometra and pyometra. Samples of the endometrium of healthy canine females were obtained in the right-handed stage, without any alteration or affection in the reproductive tract or in other systems (Group 1, n = 39) and endometrial samples of canine females affected by mucometra (Group 2, n = 43), and canine females affected by pyometra (Pyometra Group, Group 3 to 6, n = 118) during elective or therapeutic ovariohysterectomies (OH). The data were also compared among the groups classified according to the ASA (American Society of Anesthesiologists) classification. Anamnesis of the detailed reproductive history, physical examination, vulvar macroscopy examination, vaginal cytology, hematological and biochemical exams, and serum progesterone and estradiol dosage, imaging (radiography and abdominal ultrasonography) were performed. The total protein concentration of the blood serum was determined by the biuret method. The sample readings were performed in semi-automatic spectrophotometer (Labquest, Labtest, Belo Horizonte, Minas Gerais, Brazil), with specific wavelengths for the test. SDS-PAGE was used for protein fractionation. Serum levels of the acute phase proteins, ceruloplasmin, transferrin, albumin (p <0.0001), IgGCP (p <0.0001), haptoglobin (p <0.0001), alpha glycoprotein acid (p <0.0001), IgGCL (p <0.0001) and those identified by their atomic weight 44,000 Da, 40,000 Da (p = 0,0391) and 23,000 Da (p = 0.0031). have undergone significant changes, aiding in early prognosis and even more.
The objective of this study was to analyze the use of acute phase proteins (PFA) and biochemical-serum profile as a method to perform early differential diagnosis of mucometra and pyometra. Samples of the endometrium of healthy canine females were obtained in the right-handed stage, without any alteration or affection in the reproductive tract or in other systems (Group 1, n = 39) and endometrial samples of canine females affected by mucometra (Group 2, n = 43), and canine females affected by pyometra (Pyometra Group, Group 3 to 6, n = 118) during elective or therapeutic ovariohysterectomies (OH). The data were also compared among the groups classified according to the ASA (American Society of Anesthesiologists) classification. Anamnesis of the detailed reproductive history, physical examination, vulvar macroscopy examination, vaginal cytology, hematological and biochemical exams, and serum progesterone and estradiol dosage, imaging (radiography and abdominal ultrasonography) were performed. The total protein concentration of the blood serum was determined by the biuret method. The sample readings were performed in semi-automatic spectrophotometer (Labquest, Labtest, Belo Horizonte, Minas Gerais, Brazil), with specific wavelengths for the test. SDS-PAGE was used for protein fractionation. Serum levels of the acute phase proteins, ceruloplasmin, transferrin, albumin (p <0.0001), IgGCP (p <0.0001), haptoglobin (p <0.0001), alpha glycoprotein acid (p <0.0001), IgGCL (p <0.0001) and those identified by their atomic weight 44,000 Da, 40,000 Da (p = 0,0391) and 23,000 Da (p = 0.0031). have undergone significant changes, aiding in early prognosis and even more.
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Palavras-chave
Canina, Diestro, Inflamação, Mucometra, Piometra, Proteínas