Síntese, caracterização e efeito microbiológico de nanopartículas de sílica revestidas por prata quando associadas a resina acrílica quimicamente ativada, reembasador macio e ao glaze
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Data
2017-12-06
Autores
Lucatto, Bruna Corrêa
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo deste trabalho foi sintetizar e caracterizar nanopartículas de sílica recobertas por prata, e avaliar a influência quando incorporadas à resina acrílica quimicamente ativada, ao reembasador macio, e a um glaze, em suas características microbiológicas, microestruturais e mecânicas. Foi confeccionada, pelo método de hidrólise e condensação controlada (método de Stober), uma solução contendo nanopartículas de sílica revestidas por nanopartículas de prata em duas proporções, 10 milimols e 30 milimols, onde as mesmas foram caracterizadas. As nanopartículas de sílica incorporadas com a prata foram analisadas por energia dispersiva de raios-X integrado (EDS), microscopia eletrônica de varredura (MEV), e espalhamento de luz dinâmico (DLS). Para análise microbiológica foram confeccionadas 10 amostras cilíndricas (2mmx10mm), em resina acrílica quimicamente ativada onde as partículas foram incorporadas em duas concentrações: 2.5% e 5% com as duas molaridades diferentes 10 mM e 30 mM. Em outra situação foram confeccionadas 10 amostras cilíndricas (2mmx10mm) de reembasador macio, com concentrações de 2,5% e 5% com as duas molaridades diferentes 10 mM e 30 mM, no terceiro caso as partículas foram acrescentadas a um glaze nas duas concentrações e molaridades e aplicada sob uma amostra de reembasador macio. Uma suspensão de Candida albicans e Estreptococos mutans foi utilizada para análise de concentração inibitória mínima. Amostras retangulares em resina acrílica (n=6) de 30x10x3mm, foram confeccionadas para realização do teste de resistência a flexão de três pontos de ensaios mecânicos EMIC (Modelo DL-1000, EMIC Equipamentos e Sistemas LTDA., São José dos Pinhais - PR – Brasil), com velocidade de 1mm/min. Amostras sem a presença de nanopartículas foram confeccionadas como grupo controle. A sílica inicialmente apresentava-se como um pó de coloração branca e fina, após a sua caracterização com as partículas de Nitrato de Prata, houve alteração da coloração para um tom amarelado, aumento da sua densidade e do tamanho de suas nanopartículas. Os resultados de ação antimicrobiana foram positivos para as amostras de reembasador macio e glaze com 5% e 30 mMol de nanopartículas, já no ensaio mecânico não houve diferença significante estatisticamente entre os grupos.
The aim of this work was to synthesize and characterize silver - coated silica nanoparticles, and to evaluate the influence when incorporated into chemically activated acrylic resin, soft reliner, and glaze, in their microbiological, microstructural and mechanical characteristics. A solution containing silica nanoparticles coated by silver nanoparticles in two proportions, 10 millimols and 30 millimols, where they were characterized, was prepared by the hydrolysis and controlled condensation method (Stober method). Silica nanoparticles incorporated with silver were analyzed by integrated X-ray dispersive energy (EDS), scanning electron microscopy (SEM), and dynamic light scattering (DLS).For the microbiological analysis, 10 cylindrical samples (2mmx10mm) were made in chemically activated acrylic resin where the particles were incorporated in two concentrations: 2.5% and 5% with two different molarities of 10 mM and 30 mM. In another situation, 10 cylindrical samples (2mmx10mm) of soft reliner were made, with concentrations of 2.5% and 5% with the two different molarities 10 mM and 30 mM, in the third case the particles were added to a glaze in the two concentrations and molarities and applied under a sample of soft reliner. A suspension of Candida albicans and Streptococcus mutans was used for analysis of minimal inhibitory concentration. The samples were made in acrylic resin (n = 6) of 30x10x3mm and were made to perform the three-point flexural strength test of EMIC (Model DL-1000, EMIC Equipamentos e Sistemas Ltda., São José dos Pinhais - PR - Brazil), with a speed of 1mm / min. Samples without the presence of nanoparticles were made as a control group. The silica initially presented as a fine white powder after its characterization with the Silver Nitrate particles, there was a change of coloration to a yellowish tone, increase of its density and the size of its nanoparticles. The results of antimicrobial action were positive for the soft reliner and glaze samples with 5% and 30 mMol of nanoparticles, whereas in the mechanical assay there was no statistically significant difference between the groups.
The aim of this work was to synthesize and characterize silver - coated silica nanoparticles, and to evaluate the influence when incorporated into chemically activated acrylic resin, soft reliner, and glaze, in their microbiological, microstructural and mechanical characteristics. A solution containing silica nanoparticles coated by silver nanoparticles in two proportions, 10 millimols and 30 millimols, where they were characterized, was prepared by the hydrolysis and controlled condensation method (Stober method). Silica nanoparticles incorporated with silver were analyzed by integrated X-ray dispersive energy (EDS), scanning electron microscopy (SEM), and dynamic light scattering (DLS).For the microbiological analysis, 10 cylindrical samples (2mmx10mm) were made in chemically activated acrylic resin where the particles were incorporated in two concentrations: 2.5% and 5% with two different molarities of 10 mM and 30 mM. In another situation, 10 cylindrical samples (2mmx10mm) of soft reliner were made, with concentrations of 2.5% and 5% with the two different molarities 10 mM and 30 mM, in the third case the particles were added to a glaze in the two concentrations and molarities and applied under a sample of soft reliner. A suspension of Candida albicans and Streptococcus mutans was used for analysis of minimal inhibitory concentration. The samples were made in acrylic resin (n = 6) of 30x10x3mm and were made to perform the three-point flexural strength test of EMIC (Model DL-1000, EMIC Equipamentos e Sistemas Ltda., São José dos Pinhais - PR - Brazil), with a speed of 1mm / min. Samples without the presence of nanoparticles were made as a control group. The silica initially presented as a fine white powder after its characterization with the Silver Nitrate particles, there was a change of coloration to a yellowish tone, increase of its density and the size of its nanoparticles. The results of antimicrobial action were positive for the soft reliner and glaze samples with 5% and 30 mMol of nanoparticles, whereas in the mechanical assay there was no statistically significant difference between the groups.
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Palavras-chave
Nanopartículas de prata, Resinas acrílicas, Antifúngico, Antimicrobiano, Silver nanoparticles, Acrylic resins, Antifungal, Antimicrobial