Avaliação de parâmetros bioquímicos em órgãos de Phrynops geoffroanus (cágado-de-barbelas), expostos ao benzo[a]pireno
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Data
2018-03-22
Autores
Fernandes, Mariana Rojas
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Universidade Estadual Paulista (Unesp)
Resumo
O ambiente aquático, por ação antrópica, é o compartimento que mais recebe disposição final de poluentes. Dessa forma os organismos que os habitam, permanecem em constante exposição à ação destes contaminantes que muitas vezes possuem potencial mutagênico e carcinogênico, como os hidrocarbonetos policíclicos aromáticos (HPAs). A espécie Phrynops geoffroanus ("Cágado-deBarbelas"), é comumente encontrada em áreas com grande atividade antrópica, como rios poluídos que recebem efluentes domésticos e industriais. O objetivo deste estudo foi avaliar as respostas de parâmetros bioquímicos, a partir de tecidos armazenados provindos de estudo anterior no qual a espécime, Phrynops geoffroanus, foi utilizada como biomarcador de contaminação ambiental por exposição ao benzo[a]pireno, composto mais amplamente estudado dentre os HPAs, nas concentrações de 100 µg/kg corporal, 500 µg/kg corporal, 1000 µg/kg corporal e grupo controle, após sete dias. Foram avaliadas as atividades de EROD, BROD, PROD e GST em amostras de tecidos de baço, encéfalo, rins e testículos. Foi constatada atividade significativa para análises de GST (p=0,0010) em amostras de rins, e de EROD (p=0,0020) e PROD (p=0,0214) em amostras de testículos. Uma vez que os rins contribuem para biotransformação de xenobióticos e os testículos apresentam grande ativação metabólica, os dados demonstraram a sensibilidade e responsividade diferencial de diferentes órgãos do organismo frente ao contaminante, contribuindo para uma melhor compreensão dos mecanismos de metabolização do BaP na espécie.
The aquatic environment, by anthropic action, is the compartment that receives the final disposal of pollutants. In this way the organisms that inhabit them, remain in constant exposure to the action of these contaminants that often have mutagenic and carcinogenic potential, such as polycyclic aromatic hydrocarbons (PAHs). Phrynops geoffroanus is commonly found in areas with high anthropogenic activity, such as polluted rivers that receive domestic and industrial effluents. The objective of this study was to evaluate the response of biochemical parameters from stored tissue from a previous study in which the specimen, Phrynops geoffroanus, was used as biomarker of environmental contamination by exposure to benzo [a] pyrene, a compound most widely studied among PAHs at concentrations of 100 μg / kg body weight, 500 μg / kg body weight, 1000 μg / kg body weight and control group after seven days. The activities of EROD, BROD, PROD and GST were evaluated in samples of spleen, brain, kidney and testis tissues. Significant activity was observed for GST analyzes (p = 0.0010) in kidney samples, and for EROD (p = 0.0020) and PROD (p = 0.0214) in testis samples. Since the kidneys contribute to the biotransformation of xenobiotics and the testicles show great metabolic activation, the data demonstrated the sensitivity and differential responsiveness of different organs of the organism to the contaminant, contributing to a better understanding of the metabolism mechanisms of BaP in the species.
The aquatic environment, by anthropic action, is the compartment that receives the final disposal of pollutants. In this way the organisms that inhabit them, remain in constant exposure to the action of these contaminants that often have mutagenic and carcinogenic potential, such as polycyclic aromatic hydrocarbons (PAHs). Phrynops geoffroanus is commonly found in areas with high anthropogenic activity, such as polluted rivers that receive domestic and industrial effluents. The objective of this study was to evaluate the response of biochemical parameters from stored tissue from a previous study in which the specimen, Phrynops geoffroanus, was used as biomarker of environmental contamination by exposure to benzo [a] pyrene, a compound most widely studied among PAHs at concentrations of 100 μg / kg body weight, 500 μg / kg body weight, 1000 μg / kg body weight and control group after seven days. The activities of EROD, BROD, PROD and GST were evaluated in samples of spleen, brain, kidney and testis tissues. Significant activity was observed for GST analyzes (p = 0.0010) in kidney samples, and for EROD (p = 0.0020) and PROD (p = 0.0214) in testis samples. Since the kidneys contribute to the biotransformation of xenobiotics and the testicles show great metabolic activation, the data demonstrated the sensitivity and differential responsiveness of different organs of the organism to the contaminant, contributing to a better understanding of the metabolism mechanisms of BaP in the species.
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Benzo[a]pireno, Phrynops geoffroanus, Biotransformação, Biomarcadores, Toxicologia ambiental, Benzo[a]pyrene, Biotransformation, Biomarkers, Environmental toxicology