Investigação da ocorrência de Cryptosporidium spp. em espécies de quirópteros da cidade de Maringá, Paraná
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Data
2018-06-20
Autores
Prando, Luciana
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Universidade Estadual Paulista (Unesp)
Resumo
RESUMO – Este estudo foi elaborado com o objetivo de investigar a ocorrência de Cryptosporidium spp. em quirópteros da região sul do Brasil. Cento e quarenta e sete amostras fecais de morcegos foram colhidas na cidade de Maringá (PR) e submetidas aos processos de purificação para a microscopia e extração de DNA genômico. A microscopia óptica de luz convencional foi utilizada com o uso da coloração negativa de verde malaquita para pesquisa de oocistos de Cryptosporidium spp. e a PCR foi utilizada para a amplificação de fragmentos do gene da subunidade 18S do rRNA na detecção de Cryptosporidium spp. A presença de Cryptosporidium spp. não foi constatada nas amostras fecais dos morcegos. Esse resultado pode ser considerado bom no aspecto que os morcegos são possíveis reservatórios e consequentemente, dispersores deste patógeno, sendo estes parques visitados diariamente por um grande número de pessoas. Esta é a primeira investigação do referido protozoário em morcegos nesta região.
This study was elaborated with the objective to investigate the occurrence of Cryptosporidium spp. in chiroptera of southern Brazil. One hundred and forty-seven fecal samples of bats were collected in the city of Maringá (PR) and submitted to purification processes for microscopy and extraction of genomic DNA. Optical microscopy of conventional light with negative malachite green staining was performed to investigate oocysts of Cryptosporidium spp. and PCR for the amplification of fragments of the 18S rRNA subunit gene in the detection of Cryptosporidium spp. This coccidium was not found in faecal samples of bats. This result can be considered good in the aspect that bats are possible reservoirs and consequently dispersers of this pathogen, being these parks visited daily by a large number of people. It is the first investigation of this protozoan into bats in this region
This study was elaborated with the objective to investigate the occurrence of Cryptosporidium spp. in chiroptera of southern Brazil. One hundred and forty-seven fecal samples of bats were collected in the city of Maringá (PR) and submitted to purification processes for microscopy and extraction of genomic DNA. Optical microscopy of conventional light with negative malachite green staining was performed to investigate oocysts of Cryptosporidium spp. and PCR for the amplification of fragments of the 18S rRNA subunit gene in the detection of Cryptosporidium spp. This coccidium was not found in faecal samples of bats. This result can be considered good in the aspect that bats are possible reservoirs and consequently dispersers of this pathogen, being these parks visited daily by a large number of people. It is the first investigation of this protozoan into bats in this region
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Palavras-chave: Criptosporidiose, microscopia, oocisto, quirópteros, reação em cadeia da polimerase, Chiroptera, cryptosporidiosis, microscopy, oocyst, polymerase chain reaction