Human papillomavirus (HPV) detection in lip squamous cell carcinoma: correlation with clinical aspects and risk factors
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Data
2011-03-01
Autores
Demathe, Adriana [UNESP]
Garcia, José Fernando [UNESP]
Mattar, Neivio José
Simonato, Luciana Estevam [UNESP]
Miyahara, Glauco Issamu [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Associação Brasileira de Pós -Graduação em Saúde Coletiva
Resumo
O papilomavírus humano (HPV) está associado a um largo espectro de lesões em humanos e tem sido ligado à carcinogênese oral. O objetivo deste estudo foi investigar a presença do DNA do HPV em pacientes com carcinoma espinocelular de lábio e correlacioná-la com aspectos clínicos e fatores de risco. Foram estudados 33 pacientes com carcinoma espinocelular de lábio. Destes, 30 pacientes foram positivos para o gene da beta-globina humana e então foram testados para o DNA do HPV com uso da reação em cadeia de polimerase em duas etapas (PCR e nPCR) com os oligonucleotídeos iniciadores MY11/MY09 e GP5+/ GP6+. O DNA do HPV foi detectado em 43,33% dos 30 pacientes analisados. Não houve associação com os fatores de risco analisados.
Human papillomavirus (HPV) is associated with a wide spectrum of lesions in humans, and it has been linked to oral carcinogenesis. The aim of this study was to investigate the presence of HPV DNA in patients with lip squamous cell carcinoma and to correlate it with clinical characteristics and risk factors. We studied 33 patients with lip squamous cell carcinomas. of these, 30 were positive for human beta globin gene and tested for HPV DNA, using polymerase chain reaction in two steps (PCR and nPCR) with MY11/MY09 and GP5+/GP6+ primers. HPV DNA was detected in 43.33% of patients analyzed. There was no association with the risk factors analyzed.
Human papillomavirus (HPV) is associated with a wide spectrum of lesions in humans, and it has been linked to oral carcinogenesis. The aim of this study was to investigate the presence of HPV DNA in patients with lip squamous cell carcinoma and to correlate it with clinical characteristics and risk factors. We studied 33 patients with lip squamous cell carcinomas. of these, 30 were positive for human beta globin gene and tested for HPV DNA, using polymerase chain reaction in two steps (PCR and nPCR) with MY11/MY09 and GP5+/GP6+ primers. HPV DNA was detected in 43.33% of patients analyzed. There was no association with the risk factors analyzed.
Descrição
Palavras-chave
Epidemiologia, Investigação laboratorial, Carcinoma espinocelular, Papillomaviridae, Reação em cadeia da polimerase, Oligonucleotídeos, Epidemiology, Laboratory research, Squamous cell carcinoma, Papillomaviridae, Polimerase chain reaction, Oligonucleotides
Como citar
Revista Brasileira de Epidemiologia. Associação Brasileira de Pós -Graduação em Saúde Coletiva , v. 14, n. 1, p. 98-105, 2011.