Signaling pathways associated with the expression of inflammatory mediators activated during the course of two models of experimental periodontitis
Nenhuma Miniatura disponível
Data
2009-05-22
Autores
de Aquino, Sabrina Garcia [UNESP]
Manzolli Leite, Fabio Renato [UNESP]
Stach-Machado, Dagmar Ruth
Francisco da Silva, Juliete Aparecida
Spolidório, Luis Carlos [UNESP]
Rossa, Carlos [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Pergamon-Elsevier B.V. Ltd
Resumo
Aims: Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis.Main methods: Two models were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats were used and 30 mu g LIPS from Escherichia coli was injected twice a week into the palatal aspect of the upper molars. Ligatures were placed around lower first molars. A control group received injections of PBS on the palatal gingivae whereas no ligatures were placed on the lower molars. Samples were collected 5,15 and 30 days and processed for analysis by Western blotting and stereometry.Key findings: The ligature model was associated with rapid and transient activation of extracellular-regulated kinases (ERK) and p38 mitogen-activated protein kinase (MAPK) as well as of nuclear factor kappa B (NF-kappa B). Activation of these signaling pathways on the LPS model was delayed but sustained throughout the 30-day experimental period. Inflammatory changes induced by both models were similar; however there was a significant reduction on inflammation degree on the ligature model, which paralleled the decrease observed on the activation of the signaling pathways. Activation of signal transducer and activator of transcription (SEAT)-3 by phosphorylation of Tyrosine residues and of SPAT-5 was observed only on the ligature model.Significance: Regulation of gene expression results from the activation of signaling pathways initiated by receptor-ligand binding of external antigens and also of cytokines produced by the host immune system. Understanding the signaling pathways relevant fora given condition may provide information useful for novel therapeutic approaches. (C) 2009 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Cell signaling, Mitogen-activated protein kinase (MAPK), Chronic inflammation, Periodontal disease, Experimental models
Como citar
Life Sciences. Oxford: Pergamon-Elsevier B.V. Ltd, v. 84, n. 21-22, p. 745-754, 2009.