Topiramate effects lipolysis in 3T3-L1 adipocytes
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Data
2015-11-01
Autores
Campagnaro Martins, Gabriela Poltronieri
Souza, Camila Oliveira
Marques, Scherolin De Oliveira
Luciano, Thais Fernandes
Da Silva Pieri, Bruno Luiz
Rosa, Jose Cesar
Ramos Da Silva, Adelino Sanchez
Pauli, Jose Rodrigo
Cintra, Dennys Esper
Ropelle, Eduardo Rochete
Título da Revista
ISSN da Revista
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Editor
Spandidos Publ Ltd
Resumo
Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells were incubated in 50 mu M TPM for 30 min. The -adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non-esterified fatty acid, glycerol and incorporation of C-14-palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI-58) were assessed. The levels of glycerol and non-esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI-58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3-L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS.
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Palavras-chave
obesity, topiramate, 3T3-L1 adipocytes, lipolysis, weight loss
Como citar
Biomedical Reports. Athens: Spandidos Publ Ltd, v. 3, n. 6, p. 827-830, 2015.