Abnormal cell-cycle expression of the proteins p27, mdm2 and cathepsin B in oral squamous-cell carcinoma infected with human papillomavirus
Nenhuma Miniatura disponível
Data
2011-01-01
Autores
Mazon, Renata Cristina
Gerbelli, Thais Rovigatti
Neto, Carlos Benatti [UNESP]
Brancini de Oliveira, Maria Rita [UNESP]
Donadi, Eduardo Antonio
Guimaraes Goncalves, Maria Alice
Soares, Edson Garcia
Klay, Carla Patricia
Tersariol, Ivarne
Pinhal, Maria Aparecida
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier Gmbh, Urban & Fischer Verlag
Resumo
Since the role of human papillomavirus (HPV) infection in oral carcinogenesis is still unclear, the purpose of this study was to verify the association between the expression of p27, mdm2 and cathepsin B and by HPV-related oral lesions. Fifty-five oral biopsies were studied and HPV detection and typing (6/11, 16, 18, 31 and 33) were performed using polymerase chain reaction techniques. The distribution p27, mdm2 and cathepsin B was determined by immunohistochemistry. Twenty-one (38%) out of the 55 oral lesions tested positive for HPV, of which 6(33%) were HPV 6/11, 1 (5%) was HPV 16,14 (72%) were HPV 18 and none was HPV 33/31. Among the 55 biopsies, immunopostivity for p27, mdm2 and cathepsin B was observed in 17 (30.9%), 37 (67.2%) and 37 (67.2%), respectively. Among 21 HPV-positive oral lesions, immunopostivity of mdm2, p27 and cathepsin B was found, respectively, in 6 (33%) out of 18 benign lesions (BL), 4(22%) out of 18 potential malignant epithelial lesions (PMEL) and 11(57.9%) out of 19 malignant lesions (ML). High-risk HPV types may be associated with oral carcinoma, by cell-cycle control dysregulation, contributing to oral carcinogenesis and the overexpression of mdm2, p27 and cathepsin B. (C) 2009 Elsevier GmbH. All rights reserved.
Descrição
Palavras-chave
HPV, Oral cancer, p27, md m2, Cathepsin B, Immunohistochemistry
Como citar
Acta Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 113, n. 2, p. 109-116, 2011.