In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
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Data
2017-11-01
Autores
Mello Vieira, Ana Elisa de [UNESP]
Danelon, Marcelle [UNESP]
Camara, Danielle Mendes da [UNESP]
Rosselli, Eliana Rodrigues [UNESP]
Stock, Stuart R.
Cannon, Mark L.
Xiao, Xianghui
De Carlo, Francesco
Botazzo Delbem, Alberto Carlos [UNESP]
Título da Revista
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Editor
Univ Sao Paulo Fac Odontologia Bauru
Resumo
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (% SHR) and to calculate mineral concentration (g(HAp). cm(-3)), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher % SHR followed by ACP 3 min (p<0.001). No difference (p=0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for % SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
Descrição
Palavras-chave
X-ray microtomography, Tooth remineralization, Calcium phosphates, Fluoride, Dental enamel
Como citar
Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 6, p. 596-603, 2017.