Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane
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2016-04-01
Autores
Melloni, Maria Natalia G. [UNESP]
Melloni, Maria Leticia G. [UNESP]
Neuber, Ana Caroline
Perecin, Dilermando [UNESP]
Landell, Marcos G. de Andrade
Pinto, Luciana R.
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Springer
Resumo
Commercial sugarcane cultivars and wild species have a high ploidy level allied to a complex genome and usual chromosomal instability. There are few studies related to the standardization of cytogenetic techniques in sugarcane. This work tested the efficiency of three antimitotics (colchicine, 8-hydroxyquinoline, and trifuralin) at different concentrations on the quality of metaphases spreads from a commercial sugarcane cultivar (IAC911099-Saccharum spp.) and a wild sugarcane accession (Krakatau-S. spontaneum). Although all three antimitotics were efficient for obtaining metaphases, 8-hydroxyquinoline was the most efficient for both IAC911099 and Krakatau. The chromosome number of these two genotypes was inferred. The variation in chromosome number was 2n = 90-112 for IAC911099, with a statistical modal of 2n = 112 chromosomes while the variation in chromosome number was 2n = 90-129 chromosome with a statistical modal of 2n = 128 chromosomes for Krakatau (S. spontaneum).
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Sugarcane, Colchicine, 8-Hydroxyquinoline, Trifuralin, Cytogenetics
Como citar
Sugar Tech. New Delhi: Springer India, v. 18, n. 2, p. 222-228, 2016.