Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging
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Data
2012-01-01
Autores
Montanha, S. [UNESP]
Pratavieira, S.
Jacomassi, D. P.
Rastelli, Alessandra Nara de Souza [UNESP]
Bagnato, V. S.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Spie - Int Soc Optical Engineering
Resumo
Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1 mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem (R) solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope (R) system. For image capture a scientific CCD color camera PixelFly (R) was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab (R). Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching.
Descrição
Palavras-chave
Fluorescence Image, Photosensitizer, Photogem, Antimicrobial Photodynamic Therapy, Dentine, Diffusion Coefficient, Fick's Law
Como citar
Lasers In Dentistry Xviii. Bellingham: Spie-int Soc Optical Engineering, v. 8208, p. 7, 2012.