Proteinograma sérico de cães sadios e com linfoma obtido por eletroforese em gel de poliacrilamida (SDS-PAGE)
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Data
2009-10-01
Autores
Calazans, Sabryna Gouveia [UNESP]
Daleck, C. R. [UNESP]
Fagliari, J. J. [UNESP]
Repetti, C. F.
De Nardi, A. B.
Castro, J. H. T. [UNESP]
Fernandes, S. C. [UNESP]
César, J. R. F. [UNESP]
Rodigheri, S. M. [UNESP]
Título da Revista
ISSN da Revista
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Editor
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
Resumo
Foram avaliadas amostras de soro sanguíneo de 10 cães sadios e de 12 com linfoma, utilizando-se a eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio. Houve diferença entre as médias dos teores de proteína total de cães sadios, 7,68g/dL±0,46 e de cães com linfoma, 7,93g/dL±2,49. As concentrações de IgA e IgG não foram diferentes entre os grupos. Os teores das proteínas de pesos moleculares 142000, 110000, 52000, 49000, 24000 e 18000 dáltons foram mais elevados em cães com linfoma. Os cães com linfoma apresentaram concentrações mais elevadas de ceruloplasmina, 43,95mg/dL±18,19, e haptoglobina, 554mg/dL±449,51, e menores de albumina, 2908mg/dL±476,67, em comparação aos cães sadios (ceruloplasmina: 3,42mg/dL±7,44; haptoglobina: 94,54mg/dL±59,50 e albumina: 4207mg/dL±206,18). Conclui-se que concentrações séricas mais elevadas de ceruloplasmina e haptoglobina e menores de albumina podem estar associadas ao linfoma em cães.
Blood serum samples of ten healthy dogs and 12 dogs with lymphoma were evaluated by means of sodium dodecil sulphate-polyacrylamide gel electrophoresis. There was difference in total protein concentrations among healthy dogs, 7.68g/dL±0.46 and dogs with lymphoma, 7.93g/dL±2.49 values of immunoglobulins A and G presented no difference between groups. Serum proteins with molecular weights 142,000; 110,000; 52,000; 49,000; 24,000; and 18,000 Daltons presented increased concentrations in dogs with lymphoma. Dogs with lymphoma presented increased ceruloplasmin (43.95mg/dL±18.19) and haptoglobin (554mg/dL±449.51) values and lesser albumin concentration (2,908mg/dL±476.67) when compared to healthy dogs (ceruloplasmin: 3.42mg/dL±7.44; haptoglobin: 94.54mg/dL±59.50, and albumin: 4,207mg/dL±206.18). In conclusion, increased ceruloplasmin and haptoglobin and lesser albumin serum concentrations are possibly related to lymphoma in dogs.
Blood serum samples of ten healthy dogs and 12 dogs with lymphoma were evaluated by means of sodium dodecil sulphate-polyacrylamide gel electrophoresis. There was difference in total protein concentrations among healthy dogs, 7.68g/dL±0.46 and dogs with lymphoma, 7.93g/dL±2.49 values of immunoglobulins A and G presented no difference between groups. Serum proteins with molecular weights 142,000; 110,000; 52,000; 49,000; 24,000; and 18,000 Daltons presented increased concentrations in dogs with lymphoma. Dogs with lymphoma presented increased ceruloplasmin (43.95mg/dL±18.19) and haptoglobin (554mg/dL±449.51) values and lesser albumin concentration (2,908mg/dL±476.67) when compared to healthy dogs (ceruloplasmin: 3.42mg/dL±7.44; haptoglobin: 94.54mg/dL±59.50, and albumin: 4,207mg/dL±206.18). In conclusion, increased ceruloplasmin and haptoglobin and lesser albumin serum concentrations are possibly related to lymphoma in dogs.
Descrição
Palavras-chave
Dog, Lymphoma, serum protein, Cão, Linfoma, Proteinograma
Como citar
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 61, n. 5, p. 1044-1048, 2009.