Sperm motility, fertilization, and larval development of silver catfish (Rhamdia quelen) in copper-contaminated water
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Data
2016-05-01
Autores
Bombardelli, Robie Allan
Neumann, Giovano
De Toledo, Cesar Pereira Rebechi
Sanches, Eduardo Antônio [UNESP]
De Bastos, Denise Nascimento
De Oliveira, José Dílson Silva
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Resumo
The objective of this study was to evaluate the effect of copper-contaminated water on sperm motility, fertilization, and embryonic and larval development of silver catfish (Rhamdia quelen). A randomized experimental design with five treatments and four replicates was used. Two experiments were carried out: (1) controlled fertilization was performed under different levels of copper contamination and egg hatching was performed in clean water; and (2) copper-contaminated water was used for both fertilization and hatching assays. The time of sperm motility and sperm motility rates linearly decreased with increasing copper concentration in the water. Fertilization and hatching rates were also affected when the concentrations of copper in the water were above 0.0979 mg Cu+2 L-1 and 0.0331 mg Cu+2 L-1, respectively. Gamete exposure to levels between 15 mg Cu+2 L-1 and 60 mg Cu+2 L-1 for short periods of time negatively affected sperm motility, oocyte fertilization, and egg hatching rates. In addition, when gametes and embryos were exposed at levels above 0.03 mg Cu+2 L-1 during long periods of time, egg hatching rates were reduced, and at levels between 0.05 mg Cu+2 L-1 and 0.20 mg Cu+2 L-1 the number of abnormal larvae increased.
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Embryo, Fish, Heavy metal, Reproduction, Spermatozoa
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Semina:Ciencias Agrarias, v. 37, n. 3, p. 1667-1678, 2016.