Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells
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Data
2017-11-28
Autores
Bressel, Tatiana A. B.
De Queiroz, Jana Dara Freires
Gomes Moreira, Susana Margarida
Da Fonseca, Jéssyca T.
Filho, Edson A. [UNESP]
Guastaldi, Antônio Carlos [UNESP]
Batistuzzo De Medeiros, Silvia Regina
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Background: Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scaled patterns, and an oxide layer that can improve the osseointegration of implants, increasing their usefulness as bone implant materials. Methods: Laser beam irradiation at various fluences (132, 210, or 235 J/cm2) was used to treat commercially pure titanium discs to create complex surface topographies. The titanium discs were investigated by scanning electron microscopy, X-ray diffraction, and measurement of contact angles. The surface generated at a fluence of 235 J/cm2 was used in the biological assays. The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. CHO-k1 cells were also exposed to titanium discs in the MTT assay. Results: The best titanium surface was that produced by laser beam irradiation at 235 J/cm2 fluence. Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. Conclusions: The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. This evidence supports the potential use of laser-processed titanium surfaces as bone implant materials, and their use in regenerative medicine could promote better outcomes.
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Biocompatibility, Human umbilical cord, Laser beam (Yb-YAG), Mesenchymal stem cells, Osteoinduction, Surface modification, Titanium
Como citar
Stem Cell Research and Therapy, v. 8, n. 1, 2017.