Modeling and molecular dynamics indicate that snake venom phospholipase B-like enzymes are Ntn-hydrolases
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2018-10-01
Autores
Coronado, Mônika Aparecida [UNESP]
da Silva Olivier, Danilo [UNESP]
Eberle, Raphael Josef [UNESP]
do Amaral, Marcos Serrou
Arni, Raghuvir Krishnaswamy [UNESP]
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Resumo
Phospholipase-B-like (SVPLB-like) enzymes are present in relatively small amounts in a number of venoms, however, their biological function and mechanisms of action are un-clear. A three-dimensional model of the SVPLB-like enzyme from Crotalus adamanteus was generated by homology modeling based on the crystal structures of bovine Ntn-hydrolyases and the modeled protein possesses conserved domains characteristic of Ntn-hydrolases. Molecular dynamics simulations indicate that activation by autocatalytic cleavage results in the removal of 25 amino acids which increases accessibility to the active site. SVPLB-like enzymes possess a highly reactive cysteine and are hence amidases that to belong to the N-terminal nucleophile (Ntn) hydrolase family. The Ntn-hydrolases (N-terminal nucleophile) form a superfamily of diverse enzymes that are activated autocatalytically; wherein the N-terminal catalytic nucleophile is implicated in the cleavage of the amide bond.
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Crotalus adamanteus, Molecular dynamics, Molecular modeling, Ntn-hydrolase, Snake venom phospholipase B
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Toxicon, v. 153, p. 106-113.