Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)
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Data
2004-04-30
Autores
Sannomiya, M.
Rodrigues, C. M.
Coelho, R. G.
Santos, Lourdes Campaner dos [UNESP]
Hiruma-Lima, C. A.
Brito, ARS
Vilegas, Wagner [UNESP]
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Editor
Elsevier B.V.
Resumo
The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by H-1 NMR, C-13 NMR and UV analyses. (C) 2004 Published by Elsevier B.V.
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counter-current chromatography, Byrsonima crassa, plant materials, flavonoids, amentoflavone
Como citar
Journal of Chromatography A. Amsterdam: Elsevier B.V., v. 1035, n. 1, p. 47-51, 2004.