Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation
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2016-01-01
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Teixeira Da Silva, Vanessa De C�ssia [UNESP]
De Souza Coto, Amanda Lais [UNESP]
De Carvalho Souza, Rafael [UNESP]
Neves, Marcello Bertoldi Sanchez [UNESP]
Gomes, Eleni [UNESP]
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
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This work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC) and submerged (SmC) cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 � 0.8 U/g, and in SmC after 168 hours: 2,642 � 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 � 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 � 0.3 U/g after 240 h with 1: 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases.
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Biochemistry Research International, v. 2016.