Immunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defects
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Data
2017-04-01
Autores
Luvizuto, Eloá Rodrigues [UNESP]
de Oliveira, Júlio César Silva [UNESP]
Gomes-Ferreira, Pedro Henrique Silva [UNESP]
Pereira, Cassiano Costa Silva [UNESP]
Faverani, Leonardo Perez [UNESP]
Antoniali, Cristina [UNESP]
Okamoto, Roberta [UNESP]
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Resumo
This study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats’ calvaria. A critical-size defect (5 mm in diameter) was filled with β-TCP alone or added with that plus 5 mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing. Histological behavior and collagen type I labeling showed increased formation of the collagen matrix, leading to a higher percentage of newly formed bone and biomaterial for tissue and more total mineralization of pure TCP when compared to the other groups. The supplementation with BMP-2 promoted faster TCP remodeling; however, there was no statistically significant difference for the bone formed in both groups (P > 0.05). Collagen-matrix formation and new bone formation reached maximum levels when the defects were filled with pure TCP, even exceeding the levels from BMP-2 supplementation.
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BMP, Collagen matrix, Histologic evaluation, Immunohistochemistry
Como citar
Acta Histochemica, v. 119, n. 3, p. 302-308, 2017.