Activity of 3′-hydroxychalcone against Cryptococcus gattii and toxicity, and efficacy in alternative animal models
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Data
2017-10-01
Autores
Palanco, Ana Cerrejón [UNESP]
Lacorte Singulani, Junya De [UNESP]
Costa-Orlandi, Caroline Barcelos [UNESP]
Gullo, Fernanda Patrícia [UNESP]
Strohmayer Lourencetti, Natália Manuela [UNESP]
Gomes, Paulo César [UNESP]
Ayusso, Gabriela Miranda [UNESP]
Dutra, Luiz Antônio [UNESP]
Silva Bolzani, Vanderlan Da [UNESP]
Regasini, Luis Octávio [UNESP]
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Resumo
Aim: This work aimed to evaluate the activity of 3′-hydroxychalcone against Cryptococcus gattii in planktonic and biofilm forms and their toxicity using alternative animal models. Materials & methods: Minimum inhibitory concentration and minimum fungicide concentration were determined. Biofilm formation and the susceptibility tests were performed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[carbonyl(phenylamino)]-2H-tetrazolium hydroxide assay. Toxicity and efficacy were checked in Danio rerio and Galleria mellonella models. Results: The compound 3′-hydroxychalcone showed fungicidal activity against C. gattii in both planktonic and biofilm forms. The toxicity in zebrafish embryos revealed a low lethal concentration. In G. mellonella, the compound did not show antifungal activity and larvae toxicity. Conclusion: Because of the activity of 3′-hydroxychalcone against C. gattii in vitro, molecular modifications should be made to improve efficacy and to reduce toxicity in vivo. </inline-graphic.
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antifungal, biofilm, chalcone, cryptococcosis, Galleria mellonella, zebrafish
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Future Microbiology, v. 12, n. 13, p. 1123-1134, 2017.