Caracterização epidemiológica de rotavírus bovino dos Estados de Minas Gerais, Goiás e Mato Grosso do Sul, no período de 2006 a 2010
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Data
2018-11-19
Autores
Siqueira, Heloisa Pinto de Godoy [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A diarreia neonatal em bovinos é muito comum e resulta em importantes perdas econômicas. O rotavírus constitui um dos principais agentes relacionado à síndrome diarreica, que pode acometer também seres humanos. A ocorrência de rotavírus em bovinos leiteiros e de corte dos Estados de Minas Gerais, Goiás e Mato Grosso do Sul foi analisada por meio de dados epidemiológicos obtidos no período de 2006 a 2010, do Laboratório de Rotaviroses, da Faculdade de Ciências Agrárias e Veterinárias (FCAV/Unesp) de Jaboticabal, São Paulo. No período estudado, foram obtidas 851 amostras de fezes de bezerros, na faixa etária de um a 90 dias de idade, independentemente da manifestação de sinal clínico de diarreia. As amostras foram provenientes de 47 rebanhos leiteiros e 30 rebanhos de corte. A análise foi feita pela técnica de eletroforese em gel de poliacrilamida (PAGE) que indicou animais positivos em 29,9% (23/77) dos rebanhos e 7,1% (60/851) das amostras. Maior prevalência de animais positivos foi encontrada em amostras de bezerros de corte, com 12% (45/370). Dos casos de diarreia, 22,6% (45/199) dos bezerros foram positivos, enquanto 2,3% (15/642) foram detectados em bezerros sem diarreia. O Estado de Goiás apresentou maior prevalência de animais positivos (11,7%, 33/282), seguido por Mato Grosso do Sul (6,5%, 17/260) e Minas Gerais (3,2%, 10/309). A frequência da infecção por rotavírus foi maior em bezerros com idade inferior a 30 dias e nos meses mais chuvosos do ano. De acordo com a migração do genoma do rotavírus na PAGE foi possível identificar quatro perfis eletroforéticos distintos, característicos de rotavírus do grupo A, mostrando diferenças entre as estirpes de campo. A caracterização de amostras pela reação em cadeia da polimerase precedida de transcrição reversa (RT-PCR) detectou a presença dos genotipos G6P[5] e G6P[11] relacionados à rotavirose em bovinos. A presença da infecção por rotavírus nas regiões estudadas ressalta a importância de medidas de controle e prevenção da enfermidade, uma vez que o agente está em constante circulação nos rebanhos bovinos brasileiros.
Rotavirus is one of the main agents related to the syndrome, which can also affect humans. The occurrence of rotavirus in dairy and beef cattle from Minas Gerais, Goiás and Mato Grosso do Sul was analyzed by epidemiological data obtained from 2006 to 2010, from the Rotavirus Laboratory at FCAV / Unesp from Jaboticabal, São Paulo. During the study period, 851 faecal samples were obtained from calves, ranging from 1 to 90 days old, regardless of clinical signs of diarrhea. Samples were obtained from 47 dairy and 30 beef cattle. Analysis by the polyacrylamide gel electrophoresis technique (PAGE) indicated positive animals in 29.9% (23/77) of the herds and 7.1% (60/851) of the samples. Higher prevalence was found in samples of beef calves, with 12% (45/370) of positive animals. The cases with diarrhea, 22.6% (45/199) of the calves were positive, while 2.3% (15/642) were detected in calves without diarrhea. Goiás state had a higher prevalence of positive animals (11.7%, 33/282), followed by Mato Grosso do Sul (6.5%, 17/260) and Minas Gerais (3.2%, 10/309). The frequency of rotavirus infection was higher in calves under 30 days old in the rainiest months of the year. According to the migration of the rotavirus genome in PAGE it was possible to identify four distinct electrophoretic profiles, characteristic of Group A Rotavirus, showing differences between the field strains. The characterization of samples by the polymerase chain reaction preceded by reverse transcription (RT-PCR) detected the presence of G6P[5] and G6P[11] genotypes related to rotavirus in cattle. The presence of rotavirus infection in the studied regions underscores the importance control of diseases and prevention measures, since the agent is constantly circulating in Brazilian cattle herds.
Rotavirus is one of the main agents related to the syndrome, which can also affect humans. The occurrence of rotavirus in dairy and beef cattle from Minas Gerais, Goiás and Mato Grosso do Sul was analyzed by epidemiological data obtained from 2006 to 2010, from the Rotavirus Laboratory at FCAV / Unesp from Jaboticabal, São Paulo. During the study period, 851 faecal samples were obtained from calves, ranging from 1 to 90 days old, regardless of clinical signs of diarrhea. Samples were obtained from 47 dairy and 30 beef cattle. Analysis by the polyacrylamide gel electrophoresis technique (PAGE) indicated positive animals in 29.9% (23/77) of the herds and 7.1% (60/851) of the samples. Higher prevalence was found in samples of beef calves, with 12% (45/370) of positive animals. The cases with diarrhea, 22.6% (45/199) of the calves were positive, while 2.3% (15/642) were detected in calves without diarrhea. Goiás state had a higher prevalence of positive animals (11.7%, 33/282), followed by Mato Grosso do Sul (6.5%, 17/260) and Minas Gerais (3.2%, 10/309). The frequency of rotavirus infection was higher in calves under 30 days old in the rainiest months of the year. According to the migration of the rotavirus genome in PAGE it was possible to identify four distinct electrophoretic profiles, characteristic of Group A Rotavirus, showing differences between the field strains. The characterization of samples by the polymerase chain reaction preceded by reverse transcription (RT-PCR) detected the presence of G6P[5] and G6P[11] genotypes related to rotavirus in cattle. The presence of rotavirus infection in the studied regions underscores the importance control of diseases and prevention measures, since the agent is constantly circulating in Brazilian cattle herds.
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Palavras-chave
Diarreia, PAGE, Rotaviroses, RT-PCR