Comparação entre diferentes ondas de laser e óleo de girassol ozonizado na epitelização de enxertos cutâneos aplicados em feridas recém criadas de coelhos (Oryctolagus cuniculus)
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Data
2019-01-29
Autores
Reis Filho, Nazilton de Paula [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A enxertia cutânea é uma técnica cirúrgica simples e bastante útil para o reparo de feridas, principalmente nas quais existe dificuldade da aplicação do fechamento primário ou de outras técnicas reconstrutivas. Entretanto, para a sobrevivência do enxerto, é necessário que o leito da ferida esteja saudável e com a presença de um tecido de granulação saudável. Objetivou-se com este trabalho foi comparar diferentes ondas do laser de baixa intensidade e do óleo de girassol ozonizado como potenciais estimulantes da cicatrização em enxertos aplicados em feridas sem tecido de granulação em coelhos. Para isso, foram elaborados dois experimentos onde o primeiro comparou a laser de baixa intensidade (LBI) de onda contínua com a LBI pulsada (Capítulo 2), no qual 31 coelhos foram distribuídos em três grupos, grupo controle (GC), grupo laser onda contínua (GLC) e grupo laser onda pulsada (GLP). O segundo experimento comparou a LBI de onda contínua com a aplicação tópica de óleo de girassol ozonizado (OGO) (Capítulo 3) e 32 coelhos também foram distribuídos em três grupos, sendo grupo controle (GC), grupo laser (GL) e grupo ozônio (GO3). No primeiro experimento, os grupos GLC e GLP apresentaram evolução semelhante, com processo cicatricial satisfatório do enxerto em ambos, ao contrário do grupo GC. Tais resultados se mantiveram na avaliação microscópica, pois o GC apresentou correlação significativa com critérios ligadas à falha do enxerto. Já no segundo experimento, novamente ficou comprovado a ineficácia do leito doador sem tecido de granulação em proporcionar ambiente ideal para cicatrização dos enxertos. Os grupos GL e GO3 apresentaram evolução superior comparado ao GC, embora tenha ocorrido maceração nos enxertos dos animais do que receberam OGO, interferindo negativamente no processo de revascularização.
Cutaneous grafting is a simple and useful surgical technique for the repair of wounds, especially in which there is a difficulty in applying the primary closure, or other reconstructive technique. However, for graft survival, it is necessary a healthy granulation tissue in the wound bed. The aim of this study was to compare differents waves of low level laser therapy (LLLT) and ozonated sunflower oil as potential healing stimulators in grafts applied rabbits in fresh wounds. Two trials were carried out in which the first one compared LLLT continuous wave with LLLT pulsed (Chapter 2) in 31 rabbits divided into three groups: control group (CG), continuous wave laser group (GLC) and pulsed wave laser group (GLP). The second trial compared LLLT continuous with ozonized sunflower oil (OGO) (Chapter 3), and 32 rabbits were also divided into three groups, being control group (GC), laser group (GL) and ozone group (GO3). In the first trial, the GLC and GLP groups showed similar evolution, with a satisfactory healing process in both, unlike the CG group. These results were maintained in the microscopic evaluation since the GC showed a significant correlation with criteria related to graft failure. In the second trial, the ineffectiveness of the donor bed without granulation tissue to provide an ideal environment for healing of the grafts was again demonstrated. The GL and GO3 groups presented superior evolution compared to the CG, although maceration occurred in the grafts of the animals that received OGO, have a negative effect on the revascularization process.
Cutaneous grafting is a simple and useful surgical technique for the repair of wounds, especially in which there is a difficulty in applying the primary closure, or other reconstructive technique. However, for graft survival, it is necessary a healthy granulation tissue in the wound bed. The aim of this study was to compare differents waves of low level laser therapy (LLLT) and ozonated sunflower oil as potential healing stimulators in grafts applied rabbits in fresh wounds. Two trials were carried out in which the first one compared LLLT continuous wave with LLLT pulsed (Chapter 2) in 31 rabbits divided into three groups: control group (CG), continuous wave laser group (GLC) and pulsed wave laser group (GLP). The second trial compared LLLT continuous with ozonized sunflower oil (OGO) (Chapter 3), and 32 rabbits were also divided into three groups, being control group (GC), laser group (GL) and ozone group (GO3). In the first trial, the GLC and GLP groups showed similar evolution, with a satisfactory healing process in both, unlike the CG group. These results were maintained in the microscopic evaluation since the GC showed a significant correlation with criteria related to graft failure. In the second trial, the ineffectiveness of the donor bed without granulation tissue to provide an ideal environment for healing of the grafts was again demonstrated. The GL and GO3 groups presented superior evolution compared to the CG, although maceration occurred in the grafts of the animals that received OGO, have a negative effect on the revascularization process.
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Palavras-chave
Cicatrização, Enxertia, Laserterapia, Ozonioterapia