Efeitos da terapia com células estromais mesenquimais multipotentes em cães com encefalomielite pelo vírus da cinomose
Carregando...
Data
2019-02-21
Autores
Baldotto, Suelen Berger
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
As células estromais mesenquimais multipotentes (MSCs) são células adultas que residem em diversos tecidos do corpo e possuem capacidade proliferativa, imunomoduladora, anti-inflamatória e neuroregenerativa representado pela secreção de citocinas e fatores neurotróficos. O tecido neural possui baixa capacidade regenerativa após lesões traumáticas, inflamatórias e degenerativas, sendo alvo de estudos com uso de MSCs devido ao seu potencial terapêutico. A cinomose é uma doença viral infectocontagiosa comum em cães que acomete o sistema nervoso central refletindo em sinais clínicos neurológicos causadas por lesões desmielinizantes, resultantes da replicação viral no interior dos oligodendrócitos e de células da microglia, pela regulação do complexo de histocompatibilidade de classe I e infiltração de células inflamatórias. Nesse sentido, com o intuito de reduzir os sinais clínicos neurológicos, o objetivo desse estudo foi avaliar a eficácia terapêutica das MSCs derivadas do tecido adiposo canino pela via intratecal (IT) em cães com encefalomielite viral por cinomose. Foram utilizados 10 cães, sem raça definida, apresentando sinais neurológicos de início agudo e progressivo com diagnóstico positivo pelo teste de Reação em Cadeia pela Polimerase quantitativo (qPCR). No momento 0 (M0), foi realizado exame neurológico, teste qPCR, hemograma e bioquímica sérica. No momento 1 (M1), os animais foram submetidos à anestesia para exame de ressonância magnética (RM), seguido da colheita do líquido cefalorraquiano (LCR), e então transplante de 10x106 MSCs pela via IT. Trinta dias após o transplante no momento 2 (M2), foram realizados o exame clínico, neurológico, hemograma, bioquímico, RM e analise do LCR. De acordo com os resultados obtidos nas condições experimentais descritas conclui-se que a via intratecal para o transplante das ASCs alogênicas em cães é viável e segura, pois demonstrou ser minimamente invasiva, rápida e permite transplantar um grande número de células no espaço subaracnóideo. As ASCs alogênicas por via intratecal demonstraram ser efetivas na melhora dos sinais clínicos.
Multipotent mesenchymal stromal cells (MSCs) are adult cells that reside in various tissues of the body and have proliferative, immunomodulatory, anti-inflammatory and neurooregenerative capacity represented by secretion of cytokines and neurotrophic factors. Neural tissue has low regenerative capacity after traumatic lesions, inflammatory and degenerative, being the target of studies with the use of MSCs due to its therapeutic potential. Distemper is a common viral disease, infectious and contagious of dogs that affects the central nervous system reflecting on clinical neurological signs caused by demyelinating lesions, resulting from viral replication within the oligodendrocytes and microglial cells, by regulation of the class I histocompatibility complex and infiltration of inflammatory cells. Thus, in order to reduce neurological clinical signs, the objective of this study was to evaluate the therapeutic efficacy of MSCs derived from canine adipose tissue by the intrathecal route (IT) in dogs with viral encephalomyelitis due to distemper. Ten dogs were used, mixed breed, presenting neurological signs of acute and progressive onset with positive diagnosis by quantitative Polymerase Chain Reaction (qPCR). At time 0 (M0), neurological examination, qPCR test, blood count and serum biochemistry were performed. At the moment 1 (M1), the animals were submitted to anesthesia for magnetic resonance imaging (MRI), followed by cerebrospinal fluid (CSF) harvest, and then transplantation of 10x106 MSCs via the IT. Thirty days after transplantation at time 2 (M2), clinical, neurological, hemogram, biochemical, MRI and CSF analyzes were performed. According to results in the experimental conditions described, it is concluded that the via intrathecal for the transplantation of allogeneic ASCSs in dogs is viable and safe as it has been shown to be minimally invasive, rapid and allows to transplant a large number of cells in the subarachnoid space. Intrathecal allogeneic ASCSs have been shown to be effective in improving clinical signs.
Multipotent mesenchymal stromal cells (MSCs) are adult cells that reside in various tissues of the body and have proliferative, immunomodulatory, anti-inflammatory and neurooregenerative capacity represented by secretion of cytokines and neurotrophic factors. Neural tissue has low regenerative capacity after traumatic lesions, inflammatory and degenerative, being the target of studies with the use of MSCs due to its therapeutic potential. Distemper is a common viral disease, infectious and contagious of dogs that affects the central nervous system reflecting on clinical neurological signs caused by demyelinating lesions, resulting from viral replication within the oligodendrocytes and microglial cells, by regulation of the class I histocompatibility complex and infiltration of inflammatory cells. Thus, in order to reduce neurological clinical signs, the objective of this study was to evaluate the therapeutic efficacy of MSCs derived from canine adipose tissue by the intrathecal route (IT) in dogs with viral encephalomyelitis due to distemper. Ten dogs were used, mixed breed, presenting neurological signs of acute and progressive onset with positive diagnosis by quantitative Polymerase Chain Reaction (qPCR). At time 0 (M0), neurological examination, qPCR test, blood count and serum biochemistry were performed. At the moment 1 (M1), the animals were submitted to anesthesia for magnetic resonance imaging (MRI), followed by cerebrospinal fluid (CSF) harvest, and then transplantation of 10x106 MSCs via the IT. Thirty days after transplantation at time 2 (M2), clinical, neurological, hemogram, biochemical, MRI and CSF analyzes were performed. According to results in the experimental conditions described, it is concluded that the via intrathecal for the transplantation of allogeneic ASCSs in dogs is viable and safe as it has been shown to be minimally invasive, rapid and allows to transplant a large number of cells in the subarachnoid space. Intrathecal allogeneic ASCSs have been shown to be effective in improving clinical signs.
Descrição
Palavras-chave
Células-tronco, Morbillivirus, Sistema nervoso central, Tratamento, Sequelas, Stem cells