Caracterização superficial e eficácia antibiofilme no uso de digluconato de clorexidina a 0,12 sobre resinas utilizadas na confecção de restaurações provisórias contemporâneas, com e sem a aplicação de glaze fotopolimerizável: Estudo in situ
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Data
2019-03-08
Autores
Commar, Betina Chiarelo [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Durante a reabilitação com próteses fixas, o uso de provisórios é importante para que se alcance o sucesso clínico e a longevidade da prótese definitiva, mantendo a saúde e protegendo os tecidos dentais e periodontais. O objetivo deste estudo foi avaliar a estabilidade de cor, microdureza, rugosidade e energia livre de superfície de resinas de restaurações provisórias, com e sem a aplicação de glaze fotopolimerizável, e avaliar a eficácia antibiofilme do digluconato de clorexidina a 0,12% sobre as mesmas. Para isso, foram confeccionadas amostras de resina de Bis-Acril (Protemp 4, 3M ESPE, São Paulo, Brasil) e de resina acrílica ativada quimicamente (RAAQ) (Dencor, Artigos Odontológicos Clássico LTDA, São Paulo, Brasil), de forma que, apenas metade dessas amostras foi submetida ao polimento químico com glaze fotopolimerizável. Essas amostras foram posicionadas em dispositivos palatinos contendo 4 nichos (1 RAAQ sem glaze, 1 RAAQ com glaze, 1 Bis-Acril sem glaze, 1 Bis-Acril com glaze), utilizados por voluntários (n=20). As fases experimentais foram: Os voluntários usaram o dispositivo contendo as amostras do grupo controle, durante sete dias, sendo realizada aplicação de sacarose 30% sobre as amostras. Posteriormente, usaram o dispositivo contendo novas amostras do grupo teste, durante mais sete dias, sendo realizado tratamento com digluconato de clorexidina a 0,12% e aplicação de sacarose 30%. A seguir, determinou-se a alteração de cor, microdureza, rugosidade e energia livre de superfície, inicialmente e após o período experimental, de todas as amostras, tendo sido realizada a coleta do biofilme para posterior análise de polissacarídeos extracelulares álcali-solúveis (PEC). Os dados foram submetidos à análise de variância e ao teste Tukey (p<0,05); exceto os valores de PEC, submetidos à análise de variância e ao teste Student-Newman-Keuls (p<0,001). Os grupos teste apresentaram menor alteração de cor que seus respectivos grupos controle, com diferença estatística significante somente entre os grupos Bis-Acril sem glaze. Com relação à microdureza, os valores aumentaram para os grupos teste em relação aos seus respectivos grupos controle, com diferença estatística significante no período final, com exceção do grupo RAAQ e Bis-Acril sem glaze, que mostraram apenas um aumento e diminuição numérica, respectivamente. Comparando-se os grupos teste com seus respectivos grupos controle, os valores de rugosidade aumentaram apenas numericamente entre os grupos RAAQ sem glaze, no período final. Já na análise de energia livre de superfície, os valores aumentaram para os grupos teste em relação ao seu respectivo grupo controle, sem diferença estatística significante. Os grupos teste apresentaram redução estatisticamente significativa da quantidade de PEC formado, em relação aos grupos controle. Pode-se concluir que o enxaguatório à base de digluconato de clorexidina a 0,12% foi satisfatório neste estudo in situ, melhorando significativamente as propriedades de alteração de cor, microdureza e energia livre de superfície e proporcionou uma melhora, no entanto, não significativa para a rugosidade, contribuindo assim, para um melhor desempenho das resinas analisadas. A aplicação do glaze também contribuiu de maneira significativa para a melhora de todas as propriedades analisadas, com exceção da alteração de cor, na qual, foi prejudicial.
During rehabilitation with ceramic fragments, it is important to use provisionalrestorations, which are essential to achieve clinical success and longevity of the definitive prosthesis, maintaining the health and protecting dental and periodontal tissues. The aimof this study was to evaluate colorstability, microhardness, roughness, and surface free energy of provisional prosthesissamples, with and without the application of light-curing glaze on the surface, and to evaluate the antibiofilm efficacy of 0.12% chlorhexidine digluconate in these samples.For this, resin samples of Bis-Acryl(Protemp, 3M ESPE, Sao Paulo, Brazil) and chemically-activated acrylic resin (CAAR)(Dencor, Artigos Odontológicos Clássico LTDA, Sao Paulo, Brazil)were made. Half of these samples were submitted to chemical polishing with light-curing glaze. These samples were placed in intraoral devices, whichcontained 4 niches(1 CAARwithout glaze, 1 CAARwith glaze, 1 Bis-Acrylwithout glaze, 1 Bis-Acrylwith glaze), used by volunteers (n=20). The experimental pases were: Thevolunteersinitially used the device containing the samples from the control group for seven days, in which 30% sucrose were applied. Then, theyused the device containing the samples from the test group for anotherseven days, in which 0.12% chlorhexidine digluconate and 30% sucrose wereapplied. Next, the tests of color change, microhardness, roughness and surface energy, initially and after theexperimentais phases, of all samples, and the biofilm was collected for later analysis of alkali-soluble extracellular polysaccharides (EPS).Data were submitted totheanalysis of variance (ANOVA)and to the Tukey test(p<0.05), except fortheEPSvalues, which were submitted to the ANOVA and Student-Newman Keuls (p<0.001). The test groups had lower color alterationin relation to their respective control groups, and there was a statistically significant difference only between the Bis-Acrylgroupswithout glaze. Regarding the microhardness, the values increased for the test groups in relation to their respective control groups, with a significant statistical difference in the final period, except for the CAARgroup without glaze, which had only a numerical increase,and the Bis-Acrylgroup without glaze, in which values decreased numerically. Comparing the test groupswith theirrespective control groups, the roughness values increased only numericallyfor the CAARgroup without glaze,in the final period. In the surface energy analysis, the values statistically increased for the test groups, when compared to their respective control group. The test groupspresented a statistically significant reductionof the biofilm amountin relation to the control groups. It can be concluded that the 0.12% chlorhexidine digluconate baserinsewas clinically satisfactory, significantly improving the properties of color change, microhardness, surface energy properties,and provided non-significantimprovementofthe roughness, thus contributing to a better clinical performance of the resins analyzed. The glaze application also contributed significantly to all the analyzed properties, except for the color change,in which it was deleterious.
During rehabilitation with ceramic fragments, it is important to use provisionalrestorations, which are essential to achieve clinical success and longevity of the definitive prosthesis, maintaining the health and protecting dental and periodontal tissues. The aimof this study was to evaluate colorstability, microhardness, roughness, and surface free energy of provisional prosthesissamples, with and without the application of light-curing glaze on the surface, and to evaluate the antibiofilm efficacy of 0.12% chlorhexidine digluconate in these samples.For this, resin samples of Bis-Acryl(Protemp, 3M ESPE, Sao Paulo, Brazil) and chemically-activated acrylic resin (CAAR)(Dencor, Artigos Odontológicos Clássico LTDA, Sao Paulo, Brazil)were made. Half of these samples were submitted to chemical polishing with light-curing glaze. These samples were placed in intraoral devices, whichcontained 4 niches(1 CAARwithout glaze, 1 CAARwith glaze, 1 Bis-Acrylwithout glaze, 1 Bis-Acrylwith glaze), used by volunteers (n=20). The experimental pases were: Thevolunteersinitially used the device containing the samples from the control group for seven days, in which 30% sucrose were applied. Then, theyused the device containing the samples from the test group for anotherseven days, in which 0.12% chlorhexidine digluconate and 30% sucrose wereapplied. Next, the tests of color change, microhardness, roughness and surface energy, initially and after theexperimentais phases, of all samples, and the biofilm was collected for later analysis of alkali-soluble extracellular polysaccharides (EPS).Data were submitted totheanalysis of variance (ANOVA)and to the Tukey test(p<0.05), except fortheEPSvalues, which were submitted to the ANOVA and Student-Newman Keuls (p<0.001). The test groups had lower color alterationin relation to their respective control groups, and there was a statistically significant difference only between the Bis-Acrylgroupswithout glaze. Regarding the microhardness, the values increased for the test groups in relation to their respective control groups, with a significant statistical difference in the final period, except for the CAARgroup without glaze, which had only a numerical increase,and the Bis-Acrylgroup without glaze, in which values decreased numerically. Comparing the test groupswith theirrespective control groups, the roughness values increased only numericallyfor the CAARgroup without glaze,in the final period. In the surface energy analysis, the values statistically increased for the test groups, when compared to their respective control group. The test groupspresented a statistically significant reductionof the biofilm amountin relation to the control groups. It can be concluded that the 0.12% chlorhexidine digluconate baserinsewas clinically satisfactory, significantly improving the properties of color change, microhardness, surface energy properties,and provided non-significantimprovementofthe roughness, thus contributing to a better clinical performance of the resins analyzed. The glaze application also contributed significantly to all the analyzed properties, except for the color change,in which it was deleterious.
Descrição
Palavras-chave
Aderência bacteriana, Resinas acrílicas, Estética dentária, Placa dentária, Bacterial adhesion, Acrylic resins, Aesthetic, Dental biofilm