Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk
Nenhuma Miniatura disponível
Data
2018-11-28
Autores
Silva, Higor Oliveira [UNESP]
Santos Lima, Joyce Aparecida [UNESP]
Gamero Aguilar, Carlos Eduardo [UNESP]
Marques Rossi, Gabriel Augusto [UNESP]
Mathias, Luis Antonio [UNESP]
Centola Vidal, Ana Maria
Título da Revista
ISSN da Revista
Título de Volume
Editor
Frontiers Media Sa
Resumo
The species of the Bacillus cereus group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the clean-in-place (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject PRJNA390851), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 x 10(5) to 3.1 x 10(7) CFU/cm(-2). Results did not show significant differences (p > 0.05) for surface adhesion between the three tested categories (TOP, TCS, and TCV) or (p > 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of B. cereus s.s. biofilms on AISI 304 stainless-steel surfaces.
Descrição
Palavras-chave
peracetic acid, sodium hypochlorite, recontamination, food safety, biofilm formation
Como citar
Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 9, 11 p., 2018.