Extrato bruto de hipófise de lambari Astyanax altiparanae (Characiformes: Characidae) como indutor de desova de espécies nativas.
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Data
2019-11-12
Autores
Silva, Laíza Maria de Jesus [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Nosso principal objetivo foi testar o uso do extrato bruto de hipófise de lambari (EBHL) na reprodução induzida de Astyanax altiparanae e Prochilodus lineatus. No experimento 1, comparamos as características reprodutivas de lambaris (obtenção do EBHL) amostrados em outubro (OUT) e dezembro (DEZ). Então, fêmeas e machos (n=2500) foram distribuídos aleatoriamente em 10 viveiros escavados (3,08 peixes/m2). Assim, 1 fêmea de cada viveiro foi amostrada em OUT (n=5) e DEZ (n=5). Nos experimentos 2 e 3, foram testados EBHL de OUT e DEZ para indução hormonal de lambari em dose única. Nestes experimentos, 0,9% NaCl (C) e 6 mg. Kg-1 CP (extrato bruto de hipófise de carpa) foram usados como controle negativo e positivo, respectivamente. No experimento 4, fracionamos as doses e adicionamos o controle sem manipulação hormonal (CC). No experimento 5, novos EBHL foram obtidos como no experimento 1. No experimento 6, testamos o EBHL para indução hormonal de curimba. Desta forma, no experimento 1, as características das fêmeas doadoras de OUT e DEZ foram similares (p>0,05) indicando que EBHL pode ser coletado ambos os meses. Nos experimentos 2 e 3 observamos desempenho reprodutivo similar entre os grupos. No experimento 4, o fracionamento das doses diminuiu a heterogeneidade no desempenho (observadas nos experimentos 2 e 3) e apenas neste experimento, pelo menos uma réplica de cada tratamento apresentou desova, exceto CC. No experimento 6, EBHL promoveu a ovulação em P. lineatus. Assim, neste estudo mostramos que o EBHL pode ser usado para reprodução induzida de A. altiparanae e P. lineatus. Assim observamos que o EBHL assim como o CP, apenas intensificou alguns parâmetros reprodutivos no lambari. Já no curimba, o EBHL e o CP induziram as desovas. Portanto, A. altiparanae não se mostrou um bom modelo biológico para o desempenho reprodutivo, pois algumas fêmeas desovaram sem necessidade de indução e, mesmo com uso de CP (amplamente usado em pesquisas e por produtores), poucas fêmeas ovularam. Portanto, neste estudo definimos de forma inédita um protocolo para coleta de EBHL que permite obter hipófises de fêmeas maduras e é capaz de induzir a desova.
Our main goal was to test the lambari pituitary extract (EBHL) in the induced reproduction of Astyanax altiparanae and Prochilodus lineatus. In the experiment 1 we compared the reproductive features of lambari (to obtain the EBHL) sampled at October (OUT) and December (DEZ). Thus, females and males (n = 2500) were distributed in 10 earthen ponds (3.08 fish / m2), and then 1 female from each earthen pond was sampled in OUT (n = 5) and in DEZ (n = 5). In the experiments 2 and 3 we tested the use of EBHL from OUT and DEZ to induce the reproduction of lambari in a single dose. Moreover, 0.9% NaCl (C) and 6 mg. Kg-1 CP (carp pituitary extract) were applied as negative and positive control, respectively. In the experiment 4 we applied the fractioned doses (10% + 90%) and added one control without handling (CC). In the experiment 5 a new batch of EBHL was collected using the same methods as in Experiment 1. In the experiment 6 we tested the use of EBHL to induce the reproduction of curimba. In the experiment 1 we observed that the EBHL’s donor from OUT and DEZ showed similar characteristics (p>0.05), indicating that the EBHL can be collected in both months. In the experiments 2 and 3 the reproductive performance was similar among groups. The application of two doses (experiment 4) decreased the reproductive performance heterogeneity in relation to experiments 2 and 3, and in this experiment at least one replicate of each treatment showed spawning (except for CC). In the experiment 6 the EBHL could induce the ovulation in P. lineatus. In summary, in this study we are showing that the EBHL was able to induce the reproduction of lambari and curimba. In the case of the former, EBHL as well as CP only intensified some reproductive parameters. In the case of curimba the ovulation was induced only when we used EBHL or CP. Lambari did not prove to be a good biological model for reproductive performance, because some females spawn without induction and, even with CP (widely used in research and by fish farmers), a reduced number of females ovulated. Finally, in this study we have for the first time a protocol for EBHL collection from mature females, which could induce the ovulation in two native species.
Our main goal was to test the lambari pituitary extract (EBHL) in the induced reproduction of Astyanax altiparanae and Prochilodus lineatus. In the experiment 1 we compared the reproductive features of lambari (to obtain the EBHL) sampled at October (OUT) and December (DEZ). Thus, females and males (n = 2500) were distributed in 10 earthen ponds (3.08 fish / m2), and then 1 female from each earthen pond was sampled in OUT (n = 5) and in DEZ (n = 5). In the experiments 2 and 3 we tested the use of EBHL from OUT and DEZ to induce the reproduction of lambari in a single dose. Moreover, 0.9% NaCl (C) and 6 mg. Kg-1 CP (carp pituitary extract) were applied as negative and positive control, respectively. In the experiment 4 we applied the fractioned doses (10% + 90%) and added one control without handling (CC). In the experiment 5 a new batch of EBHL was collected using the same methods as in Experiment 1. In the experiment 6 we tested the use of EBHL to induce the reproduction of curimba. In the experiment 1 we observed that the EBHL’s donor from OUT and DEZ showed similar characteristics (p>0.05), indicating that the EBHL can be collected in both months. In the experiments 2 and 3 the reproductive performance was similar among groups. The application of two doses (experiment 4) decreased the reproductive performance heterogeneity in relation to experiments 2 and 3, and in this experiment at least one replicate of each treatment showed spawning (except for CC). In the experiment 6 the EBHL could induce the ovulation in P. lineatus. In summary, in this study we are showing that the EBHL was able to induce the reproduction of lambari and curimba. In the case of the former, EBHL as well as CP only intensified some reproductive parameters. In the case of curimba the ovulation was induced only when we used EBHL or CP. Lambari did not prove to be a good biological model for reproductive performance, because some females spawn without induction and, even with CP (widely used in research and by fish farmers), a reduced number of females ovulated. Finally, in this study we have for the first time a protocol for EBHL collection from mature females, which could induce the ovulation in two native species.
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Palavras-chave
Biotecnologia, Desempenho reprodutivo, Estradiol, Reprodução induzida, 17,20β-P, Curimba