Avaliação da vibração de corpo inteiro, isolada ou associada ao bagging de ozônio ou óleo de girassol ozonizado, na cicatrização de feridas cutâneas em ratos
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Data
2020-10-29
Autores
Ferreira, Gustavo Manea
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Universidade Estadual Paulista (Unesp)
Resumo
O estudo teve como objetivo avaliar a Vibração de Corpo Inteiro (VCI), isolada ou associada ao Bagging de ozônio ou óleo de girassol ozonizado, na cicatrização de feridas cutâneas em ratos. Foram utilizados 96 ratos Wistar hígidos com 14 semanas de idade, e massa corpórea entre 365 e 370 gramas. Feridas de 3 cm de diâmetro foram realizadas na região dorsal sob anestesia. Os ratos foram divididos por sorteio randomizado em seis grupos de 16 animais, de acordo com o tratamento da ferida e uso ou não da VCI: Grupo 1 – solução salina (0,9%); Grupo 2 –ratos submetidos à VCI; Grupo 3 (G3) – óleo de girassol ozonizado; Grupo 4 – bagging do gás de ozônio; Grupo 5 – óleo de girassol ozonizado e VCI; Grupo 6 - bagging do gás de ozônio e VCI. As variáveis analisadas incluíram: avaliação clínica, tensiométrica e histológica; e mensuração das feridas. Foi identificada uma diminuição significativa das áreas das feridas e aumento significativo da contração das feridas em todos os grupos, com menor área e maior contração nas feridas do G6. Foi evidenciado aumento significativo somente na força tensil do grupo G5. Diferenças significativas estiveram presentes no sétimo dia de tratamento em relação à intensidade do infiltrado inflamatório intenso (neutrófilos) no G1, e intensidade leve (neutrófilos e macrófagos) nos grupos G2, G4 e G6. A associação da VCI e bagging do gás ozônio ou óleo de girassol ozonizado foi mais efetivo em relação ao tratamento isolado na cicatrização de feridas cutâneas de ratos hígidos.
The study aimed to evaluate the Whole-Body Vibration (WBV), isolated or associated with ozone Bagging or ozonated sunflower oil, in the healing of skin wounds in rats. Ninety-six healthy Wistar rats aging of 14 weeks and weighing between 365 and 370 grams. Wounds of 3 cm in diameter were performed on the dorsal region under anesthesia. The rats were divided by random drawing into six groups of 16 animals, according to the treatment of the wound and the use or not of WBV: Group 1 - saline solution (0.9%); Group 2 - rats submitted to WBV; Group 3 (G3) - ozonized sunflower oil; Group 4 - ozone bagging; Group 5 - ozonated sunflower oil and WBV; Group 6 - ozone bagging and WBV. The analyzed variables included: clinical, tensiometric and histological evaluation; and measurement of wounds. Significant decrease in wound areas and significant increase in wound contraction were identified in all groups, with a smaller area and greater contraction in the wounds of G6. Significant increase in tensile strength was observed only in G5, and statical differences were present on the 7th day of treatment in relation to the intense inflammatory infiltrate (neutrophils) in G1, and mild intensity (neutrophils and macrophages) in groups G2, G4 and G6. The association of WBV and ozone bagging or ozonated sunflower oil was more effective in relation to the isolated treatment in the healing of cutaneous wounds of healthy rats.
The study aimed to evaluate the Whole-Body Vibration (WBV), isolated or associated with ozone Bagging or ozonated sunflower oil, in the healing of skin wounds in rats. Ninety-six healthy Wistar rats aging of 14 weeks and weighing between 365 and 370 grams. Wounds of 3 cm in diameter were performed on the dorsal region under anesthesia. The rats were divided by random drawing into six groups of 16 animals, according to the treatment of the wound and the use or not of WBV: Group 1 - saline solution (0.9%); Group 2 - rats submitted to WBV; Group 3 (G3) - ozonized sunflower oil; Group 4 - ozone bagging; Group 5 - ozonated sunflower oil and WBV; Group 6 - ozone bagging and WBV. The analyzed variables included: clinical, tensiometric and histological evaluation; and measurement of wounds. Significant decrease in wound areas and significant increase in wound contraction were identified in all groups, with a smaller area and greater contraction in the wounds of G6. Significant increase in tensile strength was observed only in G5, and statical differences were present on the 7th day of treatment in relation to the intense inflammatory infiltrate (neutrophils) in G1, and mild intensity (neutrophils and macrophages) in groups G2, G4 and G6. The association of WBV and ozone bagging or ozonated sunflower oil was more effective in relation to the isolated treatment in the healing of cutaneous wounds of healthy rats.
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Palavras-chave
Pele, Plataforma vibratória, Ozônio, Trauma, Vibração mecânica, Skin, Vibrating platform, Ozone, Trauma, Mechanical vibration