Diagnosis of canine brucellosis: comparison of various serologic tests and PCR

Nenhuma Miniatura disponível

Data

2019-11-21

Autores

Mol, Juliana P. S.
Guedes, Andressa C. B.
Eckstein, Camila
Quintal, Amanda P. N.
Souza, Tayse D.
Mathias, Luis A. [UNESP]
Haddad, Joao Paulo A.
Paixao, Tatiane A.
Santos, Renato L.

Título da Revista

ISSN da Revista

Título de Volume

Editor

Sage Publications Inc

Resumo

Canine brucellosis is an infectious and contagious disease associated with reproductive losses in breeding kennels. As a zoonotic disease, it poses a risk to human health, especially for veterinarians and breeders who handle materials potentially contaminated with Brucella canis. However, canine brucellosis is a neglected and underestimated disease given the difficulties in establishing a definitive diagnosis. We evaluated the frequency of detection of B. canis in 5 breeding kennels by using various serologic methods and PCR. Circulation of B. canis in these kennels was confirmed by bacterial isolation. The frequency of positive serologic results varied from 6.3% by AGID to 16.5% by dot-ELISA. There was no positive serology for smooth Brucella. PCR testing was positive in 13.9% of samples. The only detection tests with reasonable agreement were PCR and 2ME-MAT. The diagnosis of canine brucellosis remains challenging. The use of a single laboratory method, or even the use of different laboratory methods, may not be sufficient to reach a definitive diagnosis.

Descrição

Palavras-chave

breeding kennels, Brucella canis, detection methods, reproductive diseases, zoonosis

Como citar

Journal Of Veterinary Diagnostic Investigation. Thousand Oaks: Sage Publications Inc, v. 32, n. 1, p. 77-86, 2020.

Coleções