Caracterização integral do gene 16S rRNA de bactérias não esporulantes e sua ação contra Anticarsia gemmatalis Hübner 1818 (Lepidoptera, Noctuidae)
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Data
2020-11-20
Autores
Alves, Higor de Oliveira
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Universidade Estadual Paulista (Unesp)
Resumo
O Brasil é o maior produtor mundial de Soja (Glycine max), uma leguminosa extremamente importante devido a sua fonte de proteínas e óleos essenciais para humanos e animais, além de suas aplicações nos diversos ramos da indústria. A lagarta-da-soja (Anticarsia gemmatalis) é uma grande praga que acomete essa cultura, e que, atualmente, é controlada através de defensivos agrícolas químicos e também biológicos a base Bacillus thuringiensis. O objetivo deste trabalho foi realizar uma prospecção de microrganismos do solo, caracteriza-los por meio do gene 16S rRNA e realizar bioensaios para analisar a capacidade de letalidade ou subletalidade desses isolados contra as larvas de A. gemmatalis. O sequenciamento do gene marcador foi completo, abrangendo todas as regiões conservadas do mesmo para determinar a posição filogenética dos isolados. Em relação aos bioensaios, não foram notadas capacidade de letalidade e até mesmo de subletalidade que fossem consideradas significantes, tanto para as bactérias esporulantes, quanto para as bactérias não esporulantes testadas. Apesar das bactérias testadas neste trabalho não possuírem efeitos significativos de controle da praga estudada, elas possuem outras características importantes para serem estudadas e prospectadas biotecnologicamente nos diversos setores industriais.
Brazil is the world largest producer of Soy (Glycine max), an extremely important legume due to its source of proteins and essential oils for humans and animals, in addition to its applications in the various branches of industry. The velvetbean caterpillar (Anticarsia gemmatalis) is a great pest that affects this crop, and which, currently, is controlled by chemical and also biological pesticides based on Bacillus thuringiensis. The objective of this work was to prospect soil microorganisms, characterize them using the 16S rRNA gene and perform bioassays to analyze the lethality or subletality of these isolates against A. gemmatalis larvae. The sequencing of the marker gene was complete, covering all conserved regions of it to determine the phylogenetic position of the isolates. Regarding the bioassays, no lethality or even subletality capacity was found that were considered significant, both for the sporulant bacteria and for the non-sporulant bacteria tested. Although the bacteria tested in this work do not have significant effects on the control of the studied pest, they have other characteristics that are worth studying and prospecting biotechnologically in the various industrial sectors.
Brazil is the world largest producer of Soy (Glycine max), an extremely important legume due to its source of proteins and essential oils for humans and animals, in addition to its applications in the various branches of industry. The velvetbean caterpillar (Anticarsia gemmatalis) is a great pest that affects this crop, and which, currently, is controlled by chemical and also biological pesticides based on Bacillus thuringiensis. The objective of this work was to prospect soil microorganisms, characterize them using the 16S rRNA gene and perform bioassays to analyze the lethality or subletality of these isolates against A. gemmatalis larvae. The sequencing of the marker gene was complete, covering all conserved regions of it to determine the phylogenetic position of the isolates. Regarding the bioassays, no lethality or even subletality capacity was found that were considered significant, both for the sporulant bacteria and for the non-sporulant bacteria tested. Although the bacteria tested in this work do not have significant effects on the control of the studied pest, they have other characteristics that are worth studying and prospecting biotechnologically in the various industrial sectors.
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Palavras-chave
Bioensaio, Controle Biológico, Lagarta-da-soja, Bioassay, Biological control, Velvetbean caterpillar