Analysis of the prokaryotic microbiome of females and cell culture of Rhipicephalus sanguineus sensu lato and sensu stricto from Brazil
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2020-09-09
Autores
Luzzi, Mayara de Cassia
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Universidade Estadual Paulista (Unesp)
Resumo
Duas linhagens (temperada e tropical) de Rhipicephalus sanguineus são conhecidas no Brasil e em parte da América Latina, correspondendo, respectivamente, às populações do sul e do norte. Aqui investigamos o microbioma procariótico de ambas as linhagens usando uma abordagem metataxonômica baseada no gene 16S rRNA (região V4-V5), por meio do sequenciamento de nova geração na plataforma MiSeq Illumina. Para este propósito, foram selecionadas amostras de fêmeas do ambiente e cultivo primário de células embrionárias, considerando as duas linhagens conhecidas do Brasil. Este é o primeiro estudo que investiga o microbioma procariótico de células de cultura de carrapato. Os resultados mostram que muitos grupos de bactérias detectadas nas amostras são membros típicos da microbiota da pele, mucosa bucal ou trato respiratório. Verificamos que existe uma diversidade significativa de microorganismos em fêmeas e cultura de células embrionárias nas duas linhagens de R. sanguineus, com ênfase na presença de Coxiella em todas as amostras, ainda que em diferentes proporções. Possivelmente, as espécies de Coxiella presentes nas duas linhagens de carrapatos são diferentes e co-evoluíram com essas linhagens, conduzindo a diferentes padrões de interação entre carrapatos e patógenos que podem abrigar ou transmitir aos hospedeiros vertebrados.
Two lineages (temperate and tropical) of Rhipicephalus sanguineus are known in Brazil and in part of Latin America, corresponding, respectively, to the southern and northern populations. Here, we investigated the prokaryotic microbiomes of both lineages, using the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. For this purpose, we selected specimens of females from the environment and samples of primary cultures of embryonic cells, from both lineages in Brazil. This was the first study to investigate the prokaryotic microbiome of tick cell cultures. The results showed that many bacterial taxa detected in the samples studied were typical members of the normal skin, buccal mucosa or respiratory tract. We found that there was significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both the temperate and the tropical lineage, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with these lineages, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.
Two lineages (temperate and tropical) of Rhipicephalus sanguineus are known in Brazil and in part of Latin America, corresponding, respectively, to the southern and northern populations. Here, we investigated the prokaryotic microbiomes of both lineages, using the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. For this purpose, we selected specimens of females from the environment and samples of primary cultures of embryonic cells, from both lineages in Brazil. This was the first study to investigate the prokaryotic microbiome of tick cell cultures. The results showed that many bacterial taxa detected in the samples studied were typical members of the normal skin, buccal mucosa or respiratory tract. We found that there was significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both the temperate and the tropical lineage, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with these lineages, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.
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Palavras-chave
Coxiella, Carrapato-marrom-do-cão, Linhagem tropical, Linhagem temperada, Cultura de células embrionárias, Endossimbiontes