β-glucosidase from thermophilic fungus Thermoascus crustaceus: Production and industrial potential
Carregando...
Data
2021-01-01
Autores
Garbin, Andreza P.
Garcia, Nayara F.L.
Cavalheiro, Gabriela F.
Silvestre, Maria Alice
Rodrigues, André [UNESP]
DA PAZ, Marcelo F.
Fonseca, Gustavo G.
Leite, Rodrigo S. R.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium (comprising agroindustrial residues) was evaluated. The highest production of β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), was obtained by cultivating the fungus in wheat bran with 70% humidity, during 96 h at 40°C. The enzymatic activity was optimum at pH 4.5 and 65°C. β-Glucosidase maintained its catalytic activity when incubated at a pH range of 4.0-8.0 and temperature of 30-55°C. The enzyme was strongly inhibited by glucose; even when the substrate and glucose concentrations were equal, the inhibition was not reversed, suggesting a non-competitive inhibition. In the presence of up to 10% ethanol, β-glucosidase maintained its catalytic activity. In addition to β-glucosidase, the enzymatic extract showed activity of 36 U/g for endoglucanase, 256.2 U/g for xylanase, and 18.2 U/g for β-xylosidase. The results allow to conclude that the fungus T. crustaceus has considerable potential for production of β-glucosidase and xylanase when cultivated in agroindustrial residues, thereby reducing the cost of these biocatalysts.
Descrição
Palavras-chave
Cellulases, Filamentous fungus, Industrial enzymes, Solid state cultivation, Xylanases
Como citar
Anais da Academia Brasileira de Ciencias, v. 93, n. 1, 2021.