Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation
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2021-01-01
Autores
Do Nascimento, Nivaldo Ferreira
Bertolini, Rafaela Manchin [UNESP]
Lopez, Lucia Soares [UNESP]
Nakaghi, Laura Satiko Okada
Monzani, Paulo Sérgio
Senhorini, José Augusto [UNESP]
Vianna, Roberto Castro
Yasui, George Shigueki [UNESP]
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Resumo
Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.
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Aquaculture, Chromosome manipulation, Fish, Heat shock, Polyploidy
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Zygote.