Brazilian copaifera species: Antifungal activity against clinically relevant candida species, cellular target, and in vivo toxicity
Nenhuma Miniatura disponível
Data
2020-09-01
Autores
Andrade, Géssica
Orlando, Haniel Chadwick Silva
Scorzoni, Liliana [UNESP]
Pedroso, Reginaldo Santos
Abrão, Fariza
Carvalho, Marco Túlio Menezes
Veneziani, Rodrigo Cassio Sola
Ambrósio, Sérgio Ricardo
Bastos, Jairo Kenupp
Mendes-Giannini, Maria José Soares [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Plants belonging to the genus Copaifera are widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially against Candida. The Candida-infected Caenorhabditis elegans model was used to evaluate the in vitro antifungal potential of Copaifera leaf extracts and trunk oleoresins against Candida species. The Copaifera leaf extracts exhibited good antifungal activity against all Candida species, with MIC values ranging from 5.86 to 93.75 µg/mL. Both the Copaifera paupera and Copaifera reticulata leaf extracts at 46.87 µg/mL inhibited Candida glabrata biofilm formation and showed no toxicity to C. elegans. The survival of C. glabrata-infected nematodes increased at all the tested extract concentrations. Exposure to Copaifera leaf extracts markedly increased C. glabrata cell vacuolization and cell membrane damage. Therefore, Copaifera leaf extracts are potential candidates for the development of new and safe antifungal agents.
Descrição
Palavras-chave
Antifungal, C. elegans–Candida infected model, Candida, Copaifera
Como citar
Journal of Fungi, v. 6, n. 3, p. 1-12, 2020.