Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
Nenhuma Miniatura disponível
Data
2021-01-01
Autores
Santos, Catiule de Oliveira
Costa, Sidnei Ferro [UNESP]
Souza, Fabiana Santana
Mendes, Jessica Mariane Ferreira
Pinheiro, Cristiane Garboggini Melo de
Moreira, Diogo Rodrigo de Magalhães
Silva, Luciano Kalabric
Lima, Valeria Marçal Felix de [UNESP]
Oliveira, Geraldo Gileno de Sá
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.
Descrição
Palavras-chave
Como citar
PLoS ONE, v. 16, n. 1 January, 2021.