New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians
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Data
2010-09-01
Autores
Pinho, Catarina
Rocha, Sara
Carvalho, Bruno M.
Lopes, Susana
Mourao, Sofia
Vallinoto, Marcelo
Brunes, Tuliana O.
Haddad, Celio Fernando Baptista [UNESP]
Goncalves, Helena
Sequeira, Fernando
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ISSN da Revista
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Editor
Springer
Resumo
We report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura: Pelodytidae), respectively. Cross-species amplification of the squamate markers was evaluated in four genera representing two additional families, whereas for anurans three genera corresponding to three additional families were tested. Three out of the five loci were successfully sequenced in all squamate taxa tested. Cross-amplification of the six anuran markers had lower, but still significant, success. We predict these markers will be of great utility for both population genetics and phylogenetic studies.
Descrição
Palavras-chave
Anura, Squamata, Nuclear sequence markers, Introns
Como citar
Conservation Genetics Resources. Dordrecht: Springer, v. 2, p. 181-185, 2010.