Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
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Data
2005-09
Autores
Ferreira Junior, R. S. [UNESP]
Nascimento, N.
Martinez, J. C. [UNESP]
Alves, J. B.
Meira, D. A. [UNESP]
Barraviera, B. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Centro de Estudos de Venenos e Animais Peçonhentos
Resumo
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom.
Descrição
Palavras-chave
hyperimmunization, irradiation, Crotalus durissus terrificus, ELISA, serum neutralization, detoxification
Como citar
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 3, p. 299-314, 2005.