kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients
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Data
2008-11-01
Autores
Manoel-Caetano, Fernanda da Silva [UNESP]
Aparecida Carareto, Claudia Marcia [UNESP]
Borim, Aldenis Albaneze
Miyazaki, Kenji
Silva, Ana Elizabete [UNESP]
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Editor
Elsevier B.V.
Resumo
Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T cruzi kDNA minicircle gene signatures using the low-stringency single-specific-primer PCR technique in both peripheral blood and oesophageal. mucosa from chronic chagasic patients, with or without megaesophagus, atone or in combination with cardiopathy and megacolon. It was not possible to identify a uniform pattern of shared bands between blood and oesophageal mucosa samples from individuals with the same clinical. form or mixed forms, suggesting multiple T. cruzi infections with differential tissue tropism. Thus, the results indicate that there is an intense intraspecific variability in the hypervariable regions of T cruzi kDNA, which has so far made it impossible to correlate the genetic profile of this structure with the clinical manifestations of Chagas disease. (C) 2008 Royal. Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. AIL rights reserved.
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Palavras-chave
Chagas disease, Trypanosoma cruzi, Megaesophagus, kDNA minicircles, Genetic variability, LSSP-PCR
Como citar
Transactions of The Royal Society of Tropical Medicine and Hygiene. New York: Elsevier B.V., v. 102, n. 11, p. 1102-1107, 2008.